# US 2019/0170649 A1 · Transcriptome estimation device and transcriptome estimation method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0170649 A1 - Document ID: US20190170649A1 - Status: Published application - Kind: A1 - Application number: 16/210,311 - Filed: 2018-12-05 - Published: 2019-06-06 - Earliest claimed: 2017-12-06 - Assignees: The University of Tokyo - Applicants: The University of Tokyo - Inventors: Yuichi WAKAMOTO; Koseki KOBAYASHI - Web page: https://patentgrep.com/patent/US20190170649A1 ## CPC - G01N 21/65: Raman scattering - C12Q 1/6816: characterised by the detection means (C12Q1/6804 takes precedence) - G01J 3/0208: {using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction} - G01J 3/18: using diffraction elements, e.g. grating (gratings per se G02B) - G01J 3/44: Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} - G01J 3/4412: {Scattering spectrometry (particle sizing by light scattering G01N15/0205; optical velocimetry of particles G01P5/20, G01P5/26)} - G01L 1/242: {the material being an optical fibre} - G01N 2021/0143: {with internal and external computer} - G01N 2201/067: Electro-optic, magneto-optic, acousto-optic elements - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N) - G01L: MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE (weighing G01G) ## Abstract An objective cell is irradiated with laser beam of a predetermined wavelength. Only Stokes light is selected out of detected light including reflected light and scattered light of the laser beam, and a Raman scattering spectrum is obtained by dispersion of the selected Stokes light. A transcriptome of the objective cells is estimated, based on the Raman scattering spectrum. It is preferable to estimate the transcriptome of the objective cells, based on N-dimensional Raman data obtained by dimensional reduction of the Raman scattering spectrum. This configuration only needs to irradiate the objective cell with the laser beam and does not require to destroy the objective cell. As a result, this enables the transcriptome of the cell to be estimated in a short time period without destroying the cell. ## Claims ### Claim 1 A transcriptome estimation device configured to estimate a transcriptome of an objective cell, the transcriptome estimation device comprising: a laser irradiator configured to irradiate the objective cell with laser beam of a predetermined wavelength; a light selector configured to select only Stokes light out of detected light including reflected light and scattered light by radiation of the laser beam; a spectroscope configured to disperse the selected Stokes light and output a Raman scattering spectrum; and an estimator configured to estimate the transcriptome of the objective cell, based on the Raman scattering spectrum. ### Claim 2 (depends on 1) The transcriptome estimation device according to claim 1, wherein the estimator performs dimensional reduction of the Raman scattering spectrum to N-dimensional Raman data, and estimates the transcriptome of the objective cell, based on the N-dimensional Raman data. ### Claim 3 (depends on 2) The transcriptome estimation device according to claim 2, wherein the estimator applies a conversion function that is based on a linear regression relationship between the N-dimensional Raman data of cells in M different conditions and transcriptomes of the cells in the M different conditions, to estimate the transcriptome. ### Claim 4 (depends on 3) The transcriptome estimation device according to claim 3, wherein the conversion function is determined as an inverse function of a function, which is determined by linear regression of the N-dimensional Raman data of the cells in the M different conditions from the transcriptomes of the cells in the M different conditions. ### Claim 5 A transcriptome estimation method that estimates a transcriptome of an objective cell from a Raman scattering spectrum that is obtained by irradiation of the objective cell with laser beam of a predetermined wavelength, the transcriptome estimation method comprising: obtaining N-dimensional Raman data by dimensional reduction of Raman scattering spectra of cells in M different conditions, and estimating a conversion function on assumption of a linear regression relationship between transcriptomes of the cells in the M different conditions and N-dimensional Raman data of the cells in the M different conditions; and estimating the transcriptome by applying the conversion function to N-dimensional Raman data obtained by dimensional reduction of a Raman scattering spectrum of the objective cell.