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US 2019/0169676 A1Published application
Thermal reaction device and method for using the same
AssigneeFluidigm Corporation
InventorsMarc Unger, Ian D. Manger, Michael Lucero, Yong Yi, Emily Miyashita-Lin, Anja Wienecke, Geoffrey Facer
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Abstract
A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
Claims1
A method for performing an analysis of a sample comprising the steps of:
- providing a microfluidic device having a channel dimension between 0.1 and 1000 μm, and having at least one sample channel, wherein the sample channel is able to be partitioned into a plurality of separate chambers by one or more valves, said valves being formed in said microfluidic device such that a deflectable membrane(s) can be deflected into said sample channel to partition said sample channel into one or more separate chambers,
- introducing a sample having at least one amplifiable target therein into said sample chamber,
- actuating said valves to form said separate chambers so that said at least one amplifiable target is isolated into at least one chamber,
- conducting an amplification step or steps,
- detecting whether amplification occurred in one or more chambers to determine the quantity or presence of the amplifiable target within the sample.
2 more elements
Dependencies are inferred from the claim text.