# US 2019/0169669 A1 · Pheromonal receptor of spodoptera littoralis and identification of natural ligand of said receptor and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0169669 A1 - Document ID: US20190169669A1 - Status: Published application - Kind: A1 - Application number: 16/087,312 - Filed: 2017-03-22 - Published: 2019-06-06 - Earliest claimed: 2016-03-23 - Assignees: Institut National de la Recherche Agronomique; Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS) - Applicants: Institut National de la Recherche Agronomique; Sorbonne Universite; Centre National de la Recherche Scientifique (CNRS) - Inventors: Emmanuelle Jacquin-Joly; Arthur De Fouchier; Nicolas Montagne - Web page: https://patentgrep.com/patent/US20190169669A1 ## CPC - C12Q 1/025: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics (antimicrobial activity C12Q1/18)} - C07K 14/43563: {from insects} - C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)} - G01N 2500/04: Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A) - G01N 2500/10: involving cells - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The invention relates to a novel olfactory/pheromonal receptor (SlitOR5) of Spodoptera littoralis (Lepidoptera, Noctuidae) and the identification of the natural ligand of Slit OR5 and uses thereof. ## Claims ### Claim 1 A method for determining whether a candidate agent is a ligand of an isolated polypeptide which comprises an amino acid sequence having more than 60%, preferably more than 80%, identity with the amino acid sequence of SlitOR5 from Spodoptera littoralis defined in SEQ ID NO:1, said method comprising the steps of: contacting a host cell genetically engineered with an isolated nucleic acid molecule encoding said polypeptide or with a vector comprising said isolated nucleic acid molecule or cell extract from said cells or insect organisms genetically engineered to express said isolated polypeptide with the candidate agent under conditions permitting binding of said candidate agent to said SlitOR5 polypeptide, in the presence or in the absence of Z9,E11-14:Oac, detecting the presence of any such ligand bound specifically to said SlitOR5 polypeptide and measuring if this binding is a competitive binding, an uncompetitive binding or a non-competitive binding. ### Claim 2 (depends on 1) The method according to claim 1, wherein said isolated polypeptide comprises the amino acid sequence defined in SEQ ID NO:1. ### Claim 3 (depends on 1) The method according to claim 1, wherein said isolated nucleic acid molecule has more than 60%, preferably more than 80%, identity with the DNA sequence defined in SEQ ID NO:2. ### Claim 4 (depends on 3) The method according to claim 3, wherein said isolated nucleic acid molecule has the DNA sequence defined in SEQ ID NO:2. ### Claim 5 (depends on 1) The method according to claim 1, wherein said insect organism is a Drosophila organism. ### Claim 6 A method of identifying an antagonist or inverse agonist of the signaling activity of an isolated polypeptide which comprises an amino acid sequence having more than 60%, preferably more than 80%, identity with the amino acid sequence of SlitOR5 from Spodoptera littoralis defined in SEQ ID NO:1, comprising: a) contacting said SlitOR5 polypeptide with Z9,E11-14:OAc in the presence or in the absence of a candidate agent or a sample containing a candidate agent under conditions permitting the binding of said Z9,E11-14:OAc to said SlitOR5 polypeptide; and b) measuring the binding of said SlitOR5 polypeptide to said Z9,E11-14:OAc or a signaling activity of said SlitOR5 polypeptide, c) comparing the binding or the signaling activity in the presence of said candidate agent or sample with the binding, respectively the signaling activity, in the absence of said agent or sample. ### Claim 7 A method of identifying an agonist of the signaling activity of a an isolated polypeptide which comprises an amino acid sequence having more than 60%, preferably more than 80%, identity with the amino acid sequence of SlitOR5 from Spodoptera littoralis defined in SEQ ID NO:1, said method comprising: a) contacting said SlitOR5 polypeptide with a candidate agent or a sample containing a candidate agent; b) measuring a signaling activity of said SlitOR5 polypeptide in the presence of said agent or sample; and c) comparing said activity measured in the presence of said agent or sample to said activity measured in a reaction in which said SlitOR5 polypeptide is contacted with Z9,E11-14:OAc. ### Claim 8 (depends on 1) A composition comprising an agent identified according to claim 1, used for mass trapping of an insect target, to create mating disruption or for population survey, monitoring or estimation. ### Claim 9 A kit comprising an isolated SlitOR5 polypeptide which comprises an amino acid sequence having more than 60%, preferably more than 80%, identity with the amino acid sequence of SlitOR5 from Spodoptera littoralis defined in SEQ ID NO:1 or an isolated polynucleotide encoding said SlitOR5 polypeptide or a cell genetically engineered with an isolated nucleic acid molecule encoding said polypeptide or with a vector comprising said isolated nucleic acid molecule, with or without Z9,E11-14:OAc, and packaging materials therefore. ### Claim 10 (depends on 9) Use of a kit according to claim 9, for screening agonists, antagonists, inverse agonists, attractants, repellents or anti-attractant agents of an isolated SlitOR5 polypeptide which comprises an amino acid sequence having more than 60%, preferably more than 80%, identity with the amino acid sequence of SlitOR5 from Spodoptera littoralis defined in SEQ ID NO:1.