# US 2019/0169658 A1 · Bacillus aerolacticus for producing l-lactic acid or its salts from various carbon sources > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0169658 A1 - Document ID: US20190169658A1 - Status: Published application - Kind: A1 - Application number: 16/095,012 - Filed: 2016-04-22 - Published: 2019-06-06 - Earliest claimed: 2016-04-22 - Assignees: PTT Global Chemical Public Company Limited; Chulalongkorn University - Applicants: PTT Global Chemical Public Company Limited; Chulalongkorn University - Inventors: Nuttha Thongchul; Kentaro Kodama; Paphawee Nantarajit; Techanan Wikanda; Phatthanon Prasitchoke - Web page: https://patentgrep.com/patent/US20190169658A1 ## CPC - C12P 7/56: Lactic acid - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - C12N 9/2411: {Amylases} - C12R 2001/07: Bacillus - C12Y 302/01001: Alpha-amylase (3.2.1.1) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS - C12Y: ENZYMES ## Abstract The present invention relates to a novel Bacillus aerolacticus BC-001 species and a method for producing lactic acid or its salts using said bacteria, wherein the novel bacteria species can produce L-lactic acid or its salts with high optical purity from carbohydrate carbon sources, including oligosaccharide, disaccharide and monosaccharide, wherein said bacteria can grow well under an aerobic condition, tolerate to high temperature in a range of 45 to 60° C. ## Claims ### Claim 1 An isolated Bacillus aerolacticus BC-001 species (NITE BP-01943) as deposited to microorganisms depositary institute, which produces L-lactic acid or its salts. ### Claim 2 (depends on 1) A method for producing L-lactic acid or its salts, comprising the following steps: (1) cultivating the Bacillus aerolacticus species according to claim 1 to obtain a seed culture; and (2) fermenting the seed culture obtained from step (1) in a carbon source. ### Claim 3 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein the cultivation in step (1) is performed for a period of 2 to 10 hours. ### Claim 4 (depends on 3) The method for producing L-lactic acid or its salts according to claim 3, wherein the cultivation in step (1) is performed for the period of 3 to 5 hours. ### Claim 5 (depends on 4) The method for producing L-lactic acid or its salts according to claim 4, wherein the cultivation in step (1) is performed for 5 hours. ### Claim 6 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein a concentration of the Bacillus aerolacticus species in the cultivation step is in a range of 0.5 to 5% by volume. ### Claim 7 (depends on 6) The method for producing L-lactic acid or its salts according to claim 6, wherein the concentration of the Bacillus aerolacticus species in the cultivation step is in the range of 0.5 to 2% by volume. ### Claim 8 (depends on 7) The method for producing L-lactic acid or its salts according to claim 7, wherein the concentration of the Bacillus aerolacticus species in the cultivation step is 1% by volume. ### Claim 9 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein the fermentation of seed culture in step (2) is performed at a temperature in a range of 45 to 60° C. ### Claim 10 (depends on 9) The method for producing L-lactic acid or its salts according to claim 9, wherein the fermentation of seed culture in step (2) is performed at the temperature of 50° C. ### Claim 11 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein the fermentation of seed culture in step (2) is performed under a microaerobic condition. ### Claim 12 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein the carbon source is selected from fermentable sugar, starch, or a mixture thereof. ### Claim 13 (depends on 12) The method for producing L-lactic acid or its salts according to claim 12, wherein the fermentable sugar is monosaccharide selected from glucose, fructose, galactose, or a mixture thereof. ### Claim 14 (depends on 12) The method for producing L-lactic acid or its salts according to claim 12, wherein the fermentable sugar is disaccharide selected from sucrose, lactose, maltose, cellobiose, or a mixture thereof. ### Claim 15 (depends on 12) The method for producing L-lactic acid or its salts according to claim 12, wherein the fermentable sugar is trisaccharide selected from raffinose, isomaltotriose, maltotriose, nigerotriose, kestose, or a mixture thereof. ### Claim 16 (depends on 12) The method for producing L-lactic acid or its salts according to claim 12, wherein the fermentable sugar is selected from glucose, sucrose, or a mixture thereof. ### Claim 17 (depends on 12) The method for producing L-lactic acid or its salts according to claim 12, wherein the starch is liquefied starch selected from tapioca starch, corn starch, wheat starch, or potato starch that have been contacted to amylase enzyme. ### Claim 18 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein the concentration of carbon source in the fermentation of seed culture is in a range of 50 to 200 g/L. ### Claim 19 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein the fermentation of seed culture in step (2) further comprises a step of adding glucoamylase enzyme during the fermentation of seed culture. ### Claim 20 (depends on 2) The method for producing L-lactic acid or its salts according to claim 2, wherein L-lactic acid or its salts have an optical purity more than 95%. ### Claim 21 (depends on 20) The method for producing L-lactic acid or its salts according to claim 20, wherein L-lactic acid or its salts have the optical purity more than 99%.