# US 2019/0169639 A1 · CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2019/0169639 A1
- Document ID: US20190169639A1
- Status: Published application
- Kind: A1
- Application number: 16/256,615
- Filed: 2019-01-24
- Published: 2019-06-06
- Earliest claimed: 2013-11-07
- Assignees: Editas Medicine, Inc.; The Broad Institute, Inc.; University of Iowa Research Foundation; Massachusetts Instituite of Technology
- Applicants: Editas Medicine, Inc.; The Broad Institute, Inc.; The University of Iowa Research Foundation; Massachusetts Institute of Technology
- Inventors: Feng Zhang; Deborah Palestrant; Beverly Davidson; Jordi Mata-Fink; Edgardo Rodriguez; Alexis Borisy
- Web page: https://patentgrep.com/patent/US20190169639A1

## CPC

- C12N 15/902: {using homologous recombination}
- C12N 9/22: Ribonucleases {[RNase]; Deoxyribonucleases [DNase]}
- C12N 15/11: DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity}
- C12N 15/111: {General methods applicable to biologically active non-coding nucleic acids}
- C12N 15/1137: {against enzymes (viral enzymes C12N15/1131; receptors C12N15/1138)}
- C12N 15/63: Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N 2310/10: Type of nucleic acid
- C12N 2310/20: involving clustered regularly interspaced short palindromic repeats [CRISPR]
- C12N 2800/80: Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Disclosed herein are methods and compositions useful in targeting a payload to, or editing a target nucleic acid, where a governing gRNA molecule is used to target, optionally inactivate, a Cas9 molecule or a Cas9 molecule/gRNA complex.

## Claims

### Claim 1

A gRNA molecule that targets a Cas9 molecule.