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US 2019/0169596 A1Published application
Methods and compositions for genome editing via haploid induction
AssigneeMonsanto Technology LLC
InventorsCharles L. ARMSTRONG, Edward J. Cargill, Fenggao Dong, Jonathan C. Lamb, Huachun W. Larue, Richard J. Lawrence, Thomas S. Ream
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Abstract
Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.
Claims14
1-114. (canceled)
A method of editing maize genomic DNA, comprising:
- a) introducing into the genome of a maize haploid inducer line a nucleic acid sequence encoding at least one Genome Editing Component (GEC) to produce a first maize plant;
- b) providing a second maize plant, wherein the second maize plant comprises the maize plant genomic DNA which is to be edited;
- c) pollinating the second maize plant with pollen from the first maize plant;
- and
- d) selecting at least one haploid progeny produced by the pollination of step (c) wherein the haploid progeny comprises the genome of the second maize plant but not the first maize plant, and the genome of the haploid progeny has been modified by the at least one GEC.
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