Novel glycosyl hydrolase enzymes and uses thereof
AssigneeDanisco US Inc.
InventorsBenjamin Bower, Mark Emptage, William D. Hitz, Megan Yee Hsi, Thijs Kaper, Bradley R. Kelemen, Steven Kim, Suzanne E. Lantz, Edmund A. Larenas, Colin Mitchinson, Keith Dumont Wing
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Abstract
The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
Claims138
A non-naturally occurring composition comprising:
- a. a first polypeptide or a variant thereof having β-xylosidase activity, L-α-arabinofuranosidase activity, or both β-xylosidase activity and L-α-arabinofuranosidase activity;
- and
- b. a second polypeptide or a variant thereof having xylanase activity.
An isolated, synthetic, or recombiant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:28, or to the amino acid sequence corresponding to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to the amino acid sequence corresponding to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444, (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to the amino acid sequence corresponding to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to the amino acid sequence corresponding to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or (iv) 145-439 of SEQ ID NO:8, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to the amino acid sequence corresponding to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, (vi) 303-449, (vii) 321-448, or (viii) 321-449 of SEQ ID NO:10, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to the amino acid sequence corresponding to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12, which has β-xylosidase and/or L-α-arabinofuranosidase activities.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to the amino acid sequence corresponding to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14, which has β-xylosidase and/or L-α-arabinofuranosidase activities.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to the amino acid sequence corresponding to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90%% sequence identity to the amino acid sequence of SEQ ID NO:18, or to the amino acid sequence corresponding to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:20, or to the amino acid sequence corresponding to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20, which has L-α-arabinofuranosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to the amino acid sequence corresponding to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22, which has L-α-arabinofuranosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to the amino acid sequence corresponding to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (iv) 73-622 of SEQ ID NO:2, which has β-xylosidase activity.
An isolated, synthetic, or recombinant nucleic acid encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to the amino acid sequence corresponding to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32, which has L-α-arabinofuranosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:28, or to the amino acid sequence corresponding to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to the amino acid sequence corresponding to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444, (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to the amino acid sequence corresponding to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to the amino acid sequence corresponding to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or (iv) 145-439 of SEQ ID NO:8, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to the amino acid sequence corresponding to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, (vi) 303-449, (vii) 321-448, or (viii) 321-449 of SEQ ID NO:10, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to the amino acid sequence corresponding to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12, which has β-xylosidase and/or L-α-arabinofuranosidase activities.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to the amino acid sequence corresponding to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14, which has β-xylosidase and/or L-α-arabinofuranosidase activities.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to the amino acid sequence corresponding to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to the amino acid sequence corresponding to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:20, or to the amino acid sequence corresponding to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20, which has L-α-arabinofuranosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to the amino acid sequence corresponding to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22, which has L-α-arabinofuranosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to the amino acid sequence corresponding to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (iv) 73-622 of SEQ ID NO:2, which has β-xylosidase activity.
An isolated polypeptide, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to the amino acid sequence corresponding to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32, which has L-α-arabinofuranosidase activity.
Dependencies are inferred from the claim text.