# US 2019/0169581 A1 · Human Serum Albumin Binding Compounds and Fusion Proteins Thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0169581 A1 - Document ID: US20190169581A1 - Status: Published application - Kind: A1 - Application number: 15/714,444 - Filed: 2017-09-25 - Published: 2019-06-06 - Earliest claimed: 2013-01-03 - Assignees: Covagen AG - Applicants: Covagen AG - Inventors: Fabian Buller; Ulrich Wüllner; Irene Zbinden; Isabella Attinger-Toller; Ulrike Von Der Bey; Susann König-Friedrich; Julian Bertschinger; Dragan Grabulovski; Patricia Henne - Web page: https://patentgrep.com/patent/US20190169581A1 ## CPC - C12N 9/12: transferring phosphorus containing groups, e.g. kinases (2.7) - A61P 5/00: Drugs for disorders of the endocrine system - A61P 7/00: Drugs for disorders of the blood or the extracellular fluid - A61P 7/02: Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors - A61P 35/00: Antineoplastic agents - A61P 37/02: Immunomodulators - C07K 14/47: from mammals - C07K 14/765: Serum albumin, e.g. HSA - C07K 16/18: against material from animals or humans - C07K 2317/31: multispecific - C07K 2319/31: fusions, other than Fc, for prolonged plasma life, e.g. albumin - C12Y 207/10002: Non-specific protein-tyrosine kinase (2.7.10.2), i.e. spleen tyrosine kinase - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12Y: ENZYMES ## Abstract The present invention relates to a polypeptide binding to human serum albumin and comprising or consisting of an amino acid sequence selected from the group consisting of: (a) GVTLFVALYDY(X1)(X2)(X3)(X4)(X5)(X6)D(X7)SFHKGEKFQIL(X8)(X9)(X10)(X11)(X12)G(X13)(X14)W(X15)(X16)RSLTTG(X17)(X18)G(X19)IPSNYVAPVDSIQ (SEQ ID NO: 1), wherein (X1) is A, V, I, L, M, G, P, S, T, N, Q, C, R, H, K, D or E; (X2) is R, H, K, A, V, I, L, M, G, P, S, T, N, Q or C; (X3) is R, H, K, S, T, N, Q, C, F, Y, W, A, V, I, L, M, G or P; (X4) is S, T, N, Q, C, A, V, I, L, M, G, P, R, H, K, F, Y, W, D or E; (X5) is S, T, N, Q, C, D, E, F, Y, W, A, V, I, L, M, G, P, R, H or K; (X6) is F, Y, W, A, V, I, L, M, G, P, R, H, K, S, T, N, Q or C; (X7) is A, V, I, L, M, G, P, R, H or K; (X8) is S, T, N, Q, C, D or E; (X9) is S, T, N, Q, C, D, E, A, V, I, L, M, G, P, F, Y or W; (X10) is A, V, I, L, M, G or P; (X11) is F, Y, W, R, H or K; (X12) is S, T, N, Q, C, F, Y or W; (X13) is F, Y, W, R, H, K, S, T, N, Q, C, D, E, A, V, I, L, M, G or P; (X14) is F, Y, W, A, V, I, L, M, G or P; (X15) is D, E, A, V, I, L, M, G or P; (X16) is A, V, I, L, M, G or P; (X17) is D, E, A, V, I, L, M, G, P, R, H or K; (X18) is S, T, N, Q, C, A, V, I, L, M, G or P; (X19) is F, Y, W, S, T, N, Q or C; and (b) an amino acid sequence which is at least 90% identical to the amino acid sequence of (a), wherein the identity determination excludes amino ## Claims ### Claim at position 1 -15. (canceled) ### Claim 16 A nucleic acid molecule encoding a polypeptide binding to human serum albumin, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of: SEQ ID Nos: 4 to 40. ### Claim 17 (depends on 16) The nucleic acid of claim 16, wherein the amino acid sequence is selected from the amino acid sequences of SEQ ID NOs: 4, 5 or 6 to 32. ### Claim 18 (depends on 16) A nucleic acid molecule encoding a fusion protein comprising the polypeptide of claim 16 fused to a pharmaceutically and/or diagnostically active protein or peptide. ### Claim 19 (depends on 18) The nucleic acid molecule encoding a fusion protein according to claim 18, wherein the polypeptide is fused to the pharmaceutically and/or diagnostically active protein or peptide via a linker. ### Claim 20 (depends on 18) The nucleic acid molecule encoding a fusion protein according to claim 18, wherein the pharmaceutically and/or diagnostically active protein or peptide is selected from the group consisting of a recombinant protein, an antibody, a blood factor, a hormone, an anticoagulans, a thrombolytic, a cytokine, a chemokine, and an interferon. ### Claim 21 (depends on 16, 17, 18, 19, 20) A vector comprising the nucleic acid molecule according to any one of claims 16 to 20. ### Claim 22 (depends on 16, 17, 18, 19, 20) An isolated cell comprising the nucleic acid molecule according to any one of claims 16 to 20. ### Claim 23 (depends on 21) An isolated cell comprising the vector of claim 21. ### Claim 24 (depends on 16, 18, 22) A method of producing the polypeptide encoded by the nucleic acid of claim 16 or the fusion protein of claim 18 comprising (a) culturing the isolated cell of claim 22, and (b) isolating the produced polypeptide or fusion protein. ### Claim 25 (depends on 16, 17, 23) A method of producing the polypeptide of claim 16 or the fusion protein of claim 17 comprising (a) culturing the isolated cell of claim 23, and (b) isolating the produced polypeptide or fusion protein.