# US 2019/0169574 A1 · In vitro production of pancreatic beta cells

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2019/0169574 A1
- Document ID: US20190169574A1
- Status: Published application
- Kind: A1
- Application number: 16/074,870
- Filed: 2017-01-25
- Published: 2019-06-06
- Earliest claimed: 2016-02-04
- Assignees: Nestec S.A.
- Applicants: Nestec S.A.
- Inventors: Marine Kraus; El Hadji Mamadou Dioum; Corinne Haller
- Web page: https://patentgrep.com/patent/US20190169574A1

## CPC

- A61K 35/39: Pancreas; Islets of Langerhans (Langerhans cells of epidermis A61K35/36)
- A61K 35/12: Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00)
- A61P 3/10: for hyperglycaemia, e.g. antidiabetics
- C12N 5/0676: {Pancreatic cells}
- C12N 2501/11: Epidermal growth factor [EGF]
- C12N 2501/15: Transforming growth factor beta (TGF-β)
- C12N 2501/385: of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
- C12N 2501/999: Small molecules not provided for elsewhere
- C12N 2506/45: from artificially induced pluripotent stem cells
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Use of isoxazole 9 (ISX-9) for producing a beta cell in vitro.

## Claims

### Claim at position 1

1-3. (canceled)

### Claim 4

An in vitro method for producing a beta cell comprising the step of contacting a cell with isoxazole 9 (ISX-9).

### Claim 5 (depends on 4)

The method of claim 4, wherein the cell contacted with ISX-9 is produced in vitro from a stem cell.

### Claim 6 (depends on 4)

The method of claim 4, wherein the cell contacted with ISX-9 is a pancreatic endoderm (PE) cell, an immature beta cell and a maturing beta cell.

### Claim 7 (depends on 4)

The method of claim 4, wherein the method comprises the steps:
(a) culturing a pancreatic endoderm (PE) cell for about 2-5 days, in the presence of ISX-9;
(b) culturing the cell provided by step (a) for about 4-6 days, in the absence of ISX-9; and
(c) culturing the cell provided by step (b) for about 10-14 days, in the presence of ISX-9.

### Claim 8

A beta cell producible in vitro using isoxazole 9 (ISX-9).

### Claim 9 (depends on 8)

The beta cell of claim 8, wherein the beta cell is produced by the method comprising the steps:
(a) culturing a pancreatic endoderm (PE) cell for about 2-5 days, in the presence of ISX-9;
(b) culturing the cell provided by step (a) for about 4-6 days, in the absence of ISX-9; and
(c) culturing the cell provided by step (b) for about 10-14 days, in the presence of ISX-9.

### Claim 10 (depends on 8)

The beta cell of claim 8, wherein the beta cell is produced in vitro from a stem cell and/or a pancreatic endoderm (PE) cell.

### Claim 11 (depends on 8)

The beta cell of claim 8, wherein the beta cell additionally expresses PDX1, NKX6-1, NKX2-2, NeuroD1, MafA and/or insulin.

### Claim 12 (depends on 8)

The beta cell of claim 8, wherein the beta cell is a maturing or mature beta cell.

### Claim 13 (depends on 8)

The beta cell of claim 8 for use in therapy.

### Claim 14

A method of treating or preventing diabetes comprising the step of transplanting a cell producible in vitro using isoxazole 9 (ISX-9) to a subject in need thereof.