# US 2019/0169565 A1 · Medium and method for culturing antrodia cinnamomea

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- Number: US 2019/0169565 A1
- Document ID: US20190169565A1
- Status: Published application
- Kind: A1
- Application number: 16/054,384
- Filed: 2018-08-03
- Published: 2019-06-06
- Earliest claimed: 2017-12-01
- Assignees: I-Shou University
- Applicants: I-Shou University
- Inventors: Chih-Hui YANG; Keng-Shiang Huang; Cheng-Ming Tsai; Yu-Mei Lin
- Web page: https://patentgrep.com/patent/US20190169565A1

## CPC

- C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor
- A01H 15/00: Fungi; Lichens (fungal microorganisms C12N1/14)
- C12N 2500/34: Sugars
- C12N 2500/38: Vitamins
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A medium for culturing Antrodia cinnamomea includes a carbon source, a nitrogen source, vitamin A, agar and a solvent. The medium includes 8-16 grams/liter of the carbon source, 0.4-1.6 grams/liter of the nitrogen source and 4 grams/liter of vitamin A. Moreover, a method for culturing Antrodia cinnamomea includes inoculating the medium with mycelia of Antrodia cinnamomea. The mycelia of Antrodia cinnamomea inoculated on the medium is cultured at 20-27° C. for 28-50 days.

## Claims

### Claim 1

A medium for culturing Antrodia cinnamomea, comprising:
0.8-1.6% (w/v) of a carbon source;
0.04-0.16% (w/v) of a nitrogen source;
0.4% (w/v) of vitamin A;
agar; and
a solvent until 100% (w/v).

### Claim 2 (depends on 1)

The medium as claimed in claim 1, wherein the carbon source is selected from the group consisting of malt extract, glucose, fructose, galactose, lactose, starch, cellulose, sucrose and combination therof.

### Claim 3 (depends on 1)

The medium as claimed in claim 1, wherein the nitrogen source is selected from the group consisting of peptone, yeast extract, whey protein, tryptone, amino acids, urea, ammonium acetate, ammonium chloride, ammonium nitrate, potassium nitrate, sodium nitrate and combination therof.

### Claim 4 (depends on 1)

The medium as claimed in claim 1, wherein the medium comprises 0.8% (w/v) of malt extract, 0.8% (w/v) of glucose and 0.04% (w/v) of peptone.

### Claim 5 (depends on 1)

The medium as claimed in claim 1, wherein the medium comprises 0.8% (w/v) of malt extract, 0.08% (w/v) of peptone and 0.08% (w/v) of yeast extract.

### Claim 6

A method for culturing Antrodia cinnamomea, comprising:
inoculating a medium with mycelia of Antrodia cinnamomea, wherein the medium comprises: 0.8-1.6% (w/v) of a carbon source, 0.04-0.16% (w/v) of a nitrogen source, 0.4% (w/v) of vitamin A, agar and a solvent until 100% (w/v); and
culturing the mycelia of Antrodia cinnamomea inoculated on the medium at 20-27° C. for 28-50 days.