# US 2019/0169220 A1 · Crystallization of steviol glycosides > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0169220 A1 - Document ID: US20190169220A1 - Status: Published application - Kind: A1 - Application number: 16/322,406 - Filed: 2017-08-09 - Published: 2019-06-06 - Earliest claimed: 2016-08-09 - Assignees: Dsm IP Assets B.V. - Applicants: DSM IP Assets B.V. - Inventors: Igor GALAEV; Manas Ranjan SAHOO - Web page: https://patentgrep.com/patent/US20190169220A1 ## CPC - C07H 15/256: Polyterpene radicals - A23F 3/405: {Flavouring with flavours other than natural tea flavour or tea oil} - A23F 5/465: {Flavouring with flavours other than natural coffee flavour or coffee oil} - A23L 2/60: Sweeteners - A23L 27/36: {Terpene glycosides} - C07H 1/06: Separation; Purification - C07H 1/08: from natural products - C12G 3/06: with flavouring ingredients - C12J 1/08: Addition of flavouring ingredients - A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs - A23V 2200/132: Sugar replacer - A23V 2250/258: Rebaudioside - C12G 2200/21: Wine additives, e.g. flavouring or colouring agents - C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13) - A23F: COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - C12G: WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES (beer C12C); PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H - C12J: VINEGAR; PREPARATION OR PURIFICATION THEREOF - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION ## Abstract A method for purifying rebaudioside A, which method comprises: (a) providing a solution comprising rebaudioside A; (b) concentrating the said solution; and (c) crystallizing from the solution a high purity composition comprising rebaudioside A, thereby to purify rebaudioside A. ## Claims ### Claim 1 A method for purifying rebaudioside A, which method comprises: (a) providing a solution comprising rebaudioside A; (b) concentrating said solution; and (c) crystallizing from the solution a high purity composition comprising rebaudioside A, thereby to purify rebaudioside A. ### Claim 2 (depends on 1) A method according to claim 1 for purifying a desired steviol glycoside, which method comprises: (a) providing a solution comprising rebaudioside A at a purity of at least about 20% by weight on a dry basis; (b) concentrating said solution; (c) and crystallizing from the solution a high purity composition comprising rebaudioside A, thereby to purify rebaudioside A. ### Claim 3 (depends on 1) A method according to claim 1 for purifying a desired steviol glycoside, which method comprises: (a) providing a solution comprising rebaudioside A; (b) concentrating said solution to achieve a solution comprising rebaudioside A at a concentration of at least about 80 g/L; (c) and crystallizing from the solution a high purity composition comprising rebaudioside A, thereby to purify rebaudioside A. ### Claim 4 (depends on 1) A method according to claim 1, wherein the high purity composition comprising rebaudioside A comprises rebaudioside A in a purity greater than about 60% by weight on a dry basis. ### Claim 5 (depends on 4) A method according to claim 4, wherein the high purity composition comprising rebaudioside A comprises rebaudioside A in a purity greater than about 90% by weight on a dry basis. ### Claim 6 (depends on 1) A method according to claim 1, wherein the high purity composition comprising rebaudioside A comprises at least about 98% by weight on a dry basis of total steviol glycosides. ### Claim 7 (depends on 1) A method according to claim 1, wherein the high purity composition comprising rebaudioside A comprises no more than about 150 ppm on a dry weight basis of kaurenoic acid equivalents. ### Claim 8 (depends on 1) A method according to claim 1, wherein the high purity composition comprises no more than about 2% on a dry weight basis of stevioside. ### Claim 9 (depends on 1) A method according to claim 1, wherein the concentrating (b) does not comprise chromatography to concentrate the amount of the desired steviol glycoside. ### Claim 10 (depends on 3) A method according to claim 3, wherein the concentrating (b) comprises: a combination of ultrafiltration and nanofiltration; evaporation; and/or spray-drying the solution in (a) and then redissolving the spray-dried material. ### Claim 11 (depends on 1) A method according to claim 1, wherein the high purity composition comprising rebaudioside A is crystallized from a water:organic solvent mixture. ### Claim 12 (depends on 1) A method according to claim 1, further comprising seeding the solution comprising rebaudioside A with an amount of rebaudioside A sufficient to promote crystallization of the rebaudioside A. ### Claim 13 (depends on 1) A method according to claim 1, further comprising separating and washing the high purity composition comprising rebaudioside A. ### Claim 14 (depends on 1) A method according to claim 1, further comprising drying the high purity composition comprising rebaudioside A. ### Claim 15 (depends on 1) A method according to claim 1, wherein a further purification crystallization is carried out. ### Claim 16 (depends on 1) A method according to claim 1, wherein the rebaudioside A solution comprises fermentatively-produced rebaudioside A. ### Claim 17 A method for purifying rebaudioside A, which method comprises purifying rebaudioside A in the absence of adsorption chromatography. ### Claim 18 (depends on 1) A composition comprising rebaudioside A obtainable by a method according to claim 1.