# US 2019/0169055 A1 · Method for reducing cyanuric acid in recreational water systems

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- Number: US 2019/0169055 A1
- Document ID: US20190169055A1
- Status: Published application
- Kind: A1
- Application number: 16/276,344
- Filed: 2019-02-14
- Published: 2019-06-06
- Earliest claimed: 2014-10-31
- Applicants: BiOWiSH Technologies, Inc.
- Inventors: Michael S. SHOWELL; Richard S. CARPENTER; John GORSUCH; Joseph ROBERTS; David BARTOLI; Richard ROSENE; Lora ROSENE
- Web page: https://patentgrep.com/patent/US20190169055A1

## CPC

- C02F 3/341: {Consortia of bacteria}
- C12N 1/20: Bacteria; Culture media therefor
- C02F 2101/16: Nitrogen compounds, e.g. ammonia
- C02F 2101/38: containing nitrogen
- C02F 2103/42: from bathing facilities, e.g. swimming pools
- C02F 2303/18: Removal of treatment agents after treatment
- C02F 2303/185: The treatment agent being halogen or a halogenated compound
- C02F 2305/06: Nutrients for stimulating the growth of microorganisms
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides compositions and methods of reducing cyanuric acid levels in recreational water systems.

## Claims

### Claim 1

A method for increasing clarity of water in a recreational water system, the method comprising contacting the water with a composition that includes:
(a) a mixture of Bacillus species comprising Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, and Bacillus pumilus, wherein the mixture of Bacillus species has a bacterial concentration of at least 1×106 colony forming units (CFU) per gram of the mixture, and
(b) a mixture of Lactobacillus species comprising Pediococcus acidilactici, Pediococcus pentosaceus, and Lactobacillus plantarum, wherein the mixture of Lactobacillus species has a bacterial concentration of at least 1×106 CFU per gram of the mixture.

### Claim 2 (depends on 1)

The method of claim 1, wherein the composition further comprises between 75-99% w/w of anhydrous dextrose or dextrose monohydrate.

### Claim 3 (depends on 1)

The method of claim 1, wherein the Bacillus subtilis comprises Mojavensis and/or Bacillus subtilis 34 KLB.

### Claim 4 (depends on 1)

The method of claim 1, wherein each of the Bacillus species are individually fermented aerobically, dried, and ground to an average particle size of about 200 microns.

### Claim 5 (depends on 1)

The method of claim 1, wherein each of the Lactobacillus species are fermented anaerobically, dried, and ground to an average particle size of about 200 microns.

### Claim 6 (depends on 1)

The method of claim 1, wherein the Bacillus and Lactobacillus species are non-pathogenic.

### Claim 7 (depends on 3)

The method of claim 3, wherein at least 15% of the Bacillus species are Bacillus subtilis 34 KLB.

### Claim 8 (depends on 1)

The method of claim 1, wherein each of the Lactobacillus species are present in equal amounts by weight.

### Claim 9 (depends on 1)

The method of claim 1, wherein the composition comprises at least 1% w/w of the Bacillus species.

### Claim 10 (depends on 1)

The method of claim 1, wherein the composition comprises at least 4% w/w of the Lactobacillus species.

### Claim 11 (depends on 1)

The method of claim 1, wherein the composition further comprises an inorganic mineral that stimulates bacterial respiration and growth.

### Claim 12 (depends on 11)

The method of claim 11, wherein the inorganic mineral is selected from the group consisting of disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, magnesium sulfate, calcium sulfate, magnesium chloride, calcium chloride, and iron(III) chloride.

### Claim 13 (depends on 1)

The method of claim 1, wherein the recreational water system is a swimming pool, a spa, a hot tub, or a jetted tub.

### Claim 14 (depends on 1)

The method of claim 1, wherein the composition is sprayed onto the surface of the recreational water system.

### Claim 15 (depends on 1)

The method of claim 1, wherein the composition is applied to a filter unit of the recreational water system.

### Claim 16 (depends on 1)

The method of claim 1, wherein the recreational water system is dosed by the composition at a concentration range of about 0.01 to 100 ppm.