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US 2019/0169024 A1Published application
Sequence design for efficient assembly of nucleic acid structures
AssigneePresident and Fellows of Harvard College
InventorsNikhil Gopalkrishnan, Thomas E. Schaus, Peng Yin
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Abstract
The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.
Claims32
A nucleic acid nanostructure comprising
- (a) a nucleic acid strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides;
- and
- (b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the nucleic acid strand.
An engineered nucleic acid strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides.
A kit comprising
- (a) a nucleic acid strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides;
- and
- (b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to and at least partially bound to the nucleic acid strand.
A method of producing a nucleic acid nanostructure, comprising
- incubating, in a reaction vessel,
- (a) a nucleic acid strand having a length of at least 100 nucleotides and having a nucleotide sequence that comprises less than 10% of one of C, G, T or A nucleotides, and
- (b) a plurality of oligonucleotides, wherein each oligonucleotide is at least partially complementary to the nucleic acid strand,
- under conditions that result in binding of the oligonucleotides to the nucleic acid strand to form a nucleic acid nanostructure.
1 more element
Dependencies are inferred from the claim text.