# US 2019/0168444 A1 · Method for producing gelatin formed body and gelatin formed body

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- Number: US 2019/0168444 A1
- Document ID: US20190168444A1
- Status: Published application
- Kind: A1
- Application number: 16/213,648
- Filed: 2018-12-07
- Published: 2019-06-06
- Earliest claimed: 2016-06-08
- Assignees: FUJIFILM Corporation
- Applicants: FUJIFILM Corporation
- Inventors: Manabu KATSUMURA; Takahiro HIRATSUKA; Hideo FUSHIMI; Yoshio ISHII; Ai OKAMURA; Hiroshi OTA; Jun ARAKAWA
- Web page: https://patentgrep.com/patent/US20190168444A1

## CPC

- B29C 64/112: using individual droplets, e.g. from jetting heads
- A61L 27/222: {Gelatin}
- A61L 27/3847: {Bones}
- A61L 27/50: Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials}
- A61L 27/56: Porous materials, {e.g. foams or sponges}
- B29C 64/188: involving additional operations performed on the added layers, e.g. smoothing, grinding or thickness control (surface shaping B29C59/00; after-treatment of articles without altering their shape B29C71/00)
- B29C 64/209: Heads; Nozzles
- B33Y 10/00: Processes of additive manufacturing
- B33Y 70/00: Materials specially adapted for additive manufacturing
- B33Y 80/00: Products made by additive manufacturing
- A61L 2430/02: for reconstruction of bones; weight-bearing implants
- B29L 2031/7532: {Artificial members, protheses}
- C07K 14/47: from mammals
- C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
- C12M 1/00: Apparatus for enzymology or microbiology
- B29C: SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING (making preforms B29B11/00; making laminated products by combining previously unconnected layers which become one product whose layers will remain together B32B37/00 - B32B41/00)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- B33Y: ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B29L: INDEXING SCHEME ASSOCIATED WITH SUBCLASS B29C, RELATING TO PARTICULAR ARTICLES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

An object of the present invention is to provide a method for producing a gelatin formed body having a minimized content of a component harmful to a living body and high biocompatibility with high shaping accuracy, and a gelatin formed body produced by the method. According to the present invention, provided is a method for producing a gelatin formed body, the method including: a step a of forming, on a substrate, a layer containing a powder which is obtained by air-drying an aqueous gelatin solution and has an average particle diameter of 25 to 200 μm; and a step b of jetting liquid droplets of an aqueous solution containing alcohols having a boiling point of 120° C. or lower toward the layer formed in the step a from a nozzle and flying the jetted liquid droplets so that the liquid droplets are landed on the layer formed in the step a, thereby forming a gelatin formed body.

## Claims

### Claim 1

A method for producing a gelatin formed body, the method comprising:
a step a of forming, on a substrate, a layer containing a powder which is obtained by air-drying an aqueous gelatin solution and has an average particle diameter of 25 to 200 μm; and
a step b of jetting liquid droplets of an aqueous solution containing alcohols having a boiling point of 120° C. or lower toward the layer formed in the step a from a nozzle and flying the jetted liquid droplets so that the liquid droplets are landed on the layer formed in the step a, thereby forming a gelatin formed body.

### Claim 2 (depends on 1)

The method for producing a gelatin formed body according to claim 1, wherein the gelatin in the step a is animal gelatin or recombinant gelatin.

### Claim 3 (depends on 1)

The method for producing a gelatin formed body according to claim 1, wherein the alcohol having a boiling point of 120° C. or lower in the step b is ethanol.

### Claim 4 (depends on 1)

The method for producing a gelatin formed body according to claim 1, further comprising: after the step b,
a step c of forming a layer containing a powder which is obtained by air-drying an aqueous gelatin solution and has an average particle diameter of 25 to 200 μm on the layer formed in the step a and the gelatin formed body formed in the step b; and
a step d of jetting liquid droplets of an aqueous solution containing alcohols having a boiling point of 120° C. or lower from a nozzle and flying the jetted liquid droplets so that the liquid droplets are landed on the layer formed in the step c, thereby producing a gelatin formed body.

### Claim 5 (depends on 4)

The method for producing a gelatin formed body according to claim 4, wherein the gelatin in the step c is animal gelatin or recombinant gelatin.

### Claim 6 (depends on 4)

The method for producing a gelatin formed body according to claim 4, wherein the alcohol having a boiling point of 120° C. or lower in the step d is ethanol.

### Claim 7 (depends on 1)

The method for producing a gelatin formed body according to claim 1, wherein in the step b, the produced gelatin formed body is in contact with the substrate.

### Claim 8 (depends on 1)

The method for producing a gelatin formed body according to claim 1, further comprising:
after the step b,
a step c1 of forming a layer containing a powder which is obtained by air-drying an aqueous gelatin solution and has an average particle diameter of 25 to 200 μm on the layer formed in the step a and the gelatin formed body formed in the step b; and a step d1 of jetting liquid droplets of an aqueous solution containing alcohols having a boiling point of 120° C. or lower from a nozzle and flying the jetted liquid droplets so that the liquid droplets are landed on the layer formed in the step c1, thereby producing a gelatin formed body; and
further comprising, after the step d1, a step e of forming a layer containing a powder which is obtained by air-drying an aqueous gelatin solution and has an average particle diameter of 25 to 200 μm on the layer formed in the step c1 and the gelatin formed body formed in the step d1; and a step f of jetting liquid droplets of an aqueous solution containing alcohols having a boiling point of 120° C. or lower from a nozzle and flying the jetted liquid droplets so that the liquid droplets are landed on the layer formed in the step e, thereby producing a gelatin formed body,
wherein, in the step b, the produced gelatin fainted body is in contact with the substrate,
in the step d1, the liquid droplets are landed on a region corresponding to the outer peripheral region of the upper surface of the gelatin formed body formed in the step b, and
in the step f, the liquid droplets are landed in a region corresponding to the outer peripheral region of the upper surface of the gelatin formed body formed in the step d1 and in a region inside the region surrounded by the gelatin formed body formed in the step d1.

### Claim 9 (depends on 1)

The method for producing a gelatin formed body according to claim 1, further comprising a step g of removing the powder not used for forming a gelatin formed body.

### Claim 10 (depends on 9)

The method for producing a gelatin formed body according to claim 9, further comprising a step h of heating the formed body to cure the formed body, after the step g.

### Claim 11 (depends on 10)

The method for producing a gelatin formed body according to claim 10, wherein the formed body is heated for 1 hour to 72 hours.

### Claim 12 (depends on 1)

The method for producing a gelatin formed body according to claim 1, further comprising a step i of encapsulating the gelatin formed body with a support.

### Claim 13 (depends on 1)

The method for producing a gelatin formed body according to claim 1, further comprising a step j of seeding the cells into the gelatin formed body.

### Claim 14 (depends on 1)

The method for producing a gelatin formed body according to claim 1, wherein the gelatin formed body is a scaffold for regenerative medicine or a tissue repair material.

### Claim 15 (depends on 1)

A gelatin formed body produced by the method for producing a gelatin formed body according to claim 1.

### Claim 16 (depends on 15)

The gelatin formed body according to claim 15, which is a scaffold for regenerative medicine or a tissue repair material.

### Claim 17 (depends on 15)

The gelatin formed body according to claim 15, which is a tissue repair material for bone regeneration.

### Claim 18 (depends on 15)

The gelatin formed body according to claim 15, wherein the void volume is 20% to 40%.

### Claim 19 (depends on 15)

The gelatin formed body according to claim 15, which has a pore communicating with an external space.

### Claim 20 (depends on 19)

The gelatin formed body according to claim 19, wherein the pore communicating with an external space passes through the inside of the formed body and communicates with the external space at both ends of the pore.

### Claim 21 (depends on 19)

The gelatin formed body according to claim 19, wherein a representative diameter of the pore communicating with the external space is 300 μm to 2000 μm.

### Claim 22 (depends on 15)

The gelatin formed body according to claim 15, wherein the surface is encapsulated with a support.