# US 2019/0167846 A1 · Mesenchymal stem cell-mediated functional tooth regeneration > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0167846 A1 - Document ID: US20190167846A1 - Status: Published application - Kind: A1 - Application number: 16/266,867 - Filed: 2019-02-04 - Published: 2019-06-06 - Earliest claimed: 2006-11-29 - Assignees: University of Southern California - Applicants: University of Southern California - Inventors: Songtao SHI; Wataru SONOYAMA; Takayoshi YAMAZA; Songlin WANG - Web page: https://patentgrep.com/patent/US20190167846A1 ## CPC - A61L 27/34: Macromolecular materials - A61L 27/12: Phosphorus-containing materials, e.g. apatite - A61L 27/3834: {Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells} - A61L 27/3865: {Dental/periodontal tissues} - A61L 27/3891: {as distinct cell layers} - A61C 8/0006: {Periodontal tissue or bone regeneration (support means for endoprosthetic bone substitute or bone graft implants A61F2/2846)} - A61C 8/0043: {having more than one root} - A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K) - A61C: DENTISTRY; APPARATUS OR METHODS FOR ORAL OR DENTAL HYGIENE (non-driven toothbrushes A46B; {tongue scrapers A61B17/24;} preparations for dentistry A61K6/00; preparations for cleaning the teeth or mouth A61K8/00, A61Q11/00) ## Abstract A method for reconstructing a functional tooth by using a carrier loaded with a plurality of mesenchymal stem cells including at least one of a stem cell derived from Apical Papilla (SCAP), Periodontal Ligament Stem Cells (PDLSC), and Dental Pulp Stem Cells (DPSC). The mesenchymal stem cell loaded carrier is planted into a site within a subject's oral cavity to form a bio-root upon which a crown may be affixed, The mesenchymal stem cells will develop new periodontal tissues to stabilize the bio-root. Methods disclosed herein are particularly beneficial for subjects lacking good bone structures for conventional crown treatments. ## Claims ### Claim at position 1 -6. (canceled) ### Claim 7 A stem cell composition, comprising: a plurality of human apical papilla stem cells (SCAP); wherein the SCAP are: isolated from root apical papilla of a tooth extracted from an adult human; characterized by expression of STRO-1, CD146, CD166 and CD24-positive surface markers; and capable of differentiating into odontoblastic or osteoblastic cells. ### Claim 8 (depends on 7) The composition of claim 7, further comprising human periodontal ligament stem cells (PDLSC). ### Claim 9 (depends on 7) The composition of claim 7 further comprising human dental pulp stem cells (DPSC). ### Claim 10 (depends on 7) The composition of claim 7 further comprising PDLSC and DPSC. ### Claim 11 (depends on 7) The composition of claim 7, wherein the SCAP have higher telomerase activity than that of DPSC isolated from the same human tooth. ### Claim 12 (depends on 7) The composition of claim 7, wherein the SCAP have elevated tissue regeneration capacity than that of DPSC isolated from the same human tooth. ### Claim 13 (depends on 7) The composition of claim 7, wherein the CD24 surface marker for the SCAP has higher detectability than that for DPSC isolated from the same tooth. ### Claim 14 (depends on 7) The composition of claim 7, wherein the SCAP contain higher than 0.5% CD24-positive cells. ### Claim 15 (depends on 7) The composition of claim 7, wherein the SCAP contain higher than 7.5% CD24-positive cells. ### Claim 16 (depends on 7) The composition of claim 7, wherein the SCAP are isolated from an extracted human wisdom tooth. ### Claim 17 (depends on 7) The composition of claim 7, wherein the SCAP are autologous SCAP. ### Claim 18 A stem cell composition, comprising: two stem cell compositions, wherein the first stem cell composition comprises plurality of human apical papilla stem cells (SCAP), and the second stem cell composition comprises human periodontal ligament stem cells (PDLSC); wherein the SCAP are: isolated from root apical papilla of a tooth extracted from an adult human; characterized by expression of STRO-1, CD146, CD166 and CD24-positive surface markers; and capable of differentiating into odontoblastic or osteoblastic cells. ### Claim 19 (depends on 18) The composition of claim 18, wherein the second stem cell composition further comprises a gelatin solution. ### Claim 20 (depends on 18) The composition of claim 18, wherein the SCAP have higher telomerase activity than that of DPSC isolated from the same human tooth. ### Claim 21 (depends on 18) The composition of claim 18, wherein the SCAP have elevated tissue regeneration capacity than that of DPSC isolated from the same human tooth. ### Claim 22 (depends on 18) The composition of claim 18, wherein the CD24 surface marker for the SCAP has higher detectability than that for DPSC isolated from the same tooth. ### Claim 23 (depends on 18) The composition of claim 18, wherein the SCAP contain higher than 0.5% CD24-positive cells. ### Claim 24 (depends on 18) The composition of claim 18, wherein the SCAP contain higher than 7.5% CD24-positive cells. ### Claim 25 (depends on 18) The composition of claim 18, wherein the SCAP are isolated from an extracted human wisdom tooth. ### Claim 26 (depends on 18) The composition of claim 18, wherein the SCAP are autologous SCAP.