# US 2019/0167758 A1 · Use of chelating agent and peptide antimicrobial compounds

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- Number: US 2019/0167758 A1
- Document ID: US20190167758A1
- Status: Published application
- Kind: A1
- Application number: 16/278,056
- Filed: 2019-02-16
- Published: 2019-06-06
- Earliest claimed: 2010-10-12
- Assignees: Consumo Em Verde Biotecnologia Das Plantas, S.A.
- Inventors: Alexandra Carreira; Sara Valadas Da Silva Monteiro; Ricardo De Seixas Boavida Ferreira
- Web page: https://patentgrep.com/patent/US20190167758A1

## CPC

- A61K 38/16: Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof {(enzyme inhibitors A61K38/005)}
- A01N 37/44: containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N 65/20: Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS

## Abstract

The inventors provide the use of a chelating agent and an antimicrobial agent that is effective against a plant pathogenic microorganism to inhibit the growth of and/or kill a plant pathogenic microorganism on a plant; the use of a chelating agent to increase the activity of an antimicrobial that is effective against a plant pathogenic microorganism; a method of inhibiting the growth of and/or killing a plant pathogenic microorganism comprising administering to a plant in need thereof a chelating agent and an antimicrobial agent that is effective against a plant pathogenic microorganism; and a method of increasing the activity of an antimicrobial that is effective against a plant pathogenic microorganism comprising using said antimicrobial with a chelating agent. Also provided is a composition comprising a chelating agent and an antimicrobial agent that is effective against a plant pathogenic microorganism, use of said composition to inhibit the growth of and/or kill a plant pathogenic microorganism on a plant, and a method of inhibiting the growth of and/or killing a plant pathogenic microorganism comprising administering to a plant in need thereof said composition. Further provided is the use of a composition comprising an antimicrobial polypeptide comprising Blad or an active variant thereof to kill, or inhibit the growth of, a plant pathogenic bacterium on a plant, and a method of killing, or inhibiting the growth of, a plant pathogenic bacterium on a plant, said method comprising administering to said plant a composition comprising an effective amount of an antimicrobial polypeptide comprising Blad or an active variant thereof.

## Claims

### Claim at position 1

1-22. (canceled)

### Claim 23

A method of inhibiting the growth of and/or killing a plant pathogenic microorganism, said method comprising administering to a plant a chelating agent and an antimicrobial agent that is effective against a plant pathogenic microorganism, wherein said antimicrobial agent is a polypeptide comprising the sequence shown in SEQ ID NO:4 or an active variant thereof, which has antimicrobial activity and which comprises a sequence which has at least 60% identity to either SEQ ID NO:4 or a fragment of SEQ ID NO:4.

### Claim 24

A method of increasing the activity of an antimicrobial that is effective against a plant pathogenic microorganism, said method comprising using said antimicrobial with a chelating agent, wherein said antimicrobial is a polypeptide comprising the sequence shown in SEQ ID NO:4 or an active variant thereof, which has antimicrobial activity and which comprises a sequence which has at least 60% identity to either SEQ ID NO:4 or a fragment of SEQ ID NO:4.

### Claim 25 (depends on 23)

The method according to claim 23 wherein said chelating agent and said antimicrobial agent are applied to a plant in need thereof.

### Claim 26 (depends on 25)

The method according to claim 25 wherein said antimicrobial agent and said chelating agent are administered to said plant:
a) as part of the same composition; or
b) separately, either sequentially or simultaneously.

### Claim 27 (depends on 23)

The method according to claim 23 wherein the antimicrobial agent is effective against a plant pathogenic bacterium or fungus.

### Claim 28 (depends on 23)

The method according to claim 23 wherein the chelating agent is a polyamino carboxylate.

### Claim 29 (depends on 30)

A method according to claim 30 wherein the chelating agent is EDTA.