# US 2019/0167746 A1 · Method of preparing lyophilizate of taheebo alcohol extract

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- Number: US 2019/0167746 A1
- Document ID: US20190167746A1
- Status: Published application
- Kind: A1
- Application number: 16/313,270
- Filed: 2017-07-14
- Published: 2019-06-06
- Earliest claimed: 2017-07-14
- Assignees: Nutribiotech, LLC; Nutriscience Co., Ltd.
- Applicants: Nutribiotech; Nutriscience Co., Ltd.
- Inventors: Kwang Soo BAEK; Jin Hak KIM; Su Young CHOI; Young Min PARK; Jae Seok SHIM; Sang Woo KIM; Mann Seok YOON; Yoon Jong LEE
- Web page: https://patentgrep.com/patent/US20190167746A1

## CPC

- A61K 36/53: Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A23L 33/105: Plant extracts, their artificial duplicates or their derivatives
- A61K 8/9783: Angiosperms [Magnoliophyta]
- A61K 8/9789: Magnoliopsida [dicotyledons]
- A61K 9/0014: {Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00)}
- A61K 9/19: lyophilised {, i.e. freeze-dried, solutions or dispersions (lyophilised products with subsequent particle size reduction A61K9/14; granules or pellets made by lyphilisation A61K9/1682; solid oral dosage forms made by lyophilisation A61K9/2095; lyophilisation additives A61K47/00)}
- A61K 36/185: Magnoliopsida (dicotyledons)
- A61K 47/46: Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
- A61Q 19/00: Preparations for care of the skin
- A61K 2236/00: Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61Q 17/005: {Antimicrobial preparations}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

The present invention relates to a method of preparing a lyophilizate of a Taheebo alcohol extract, including: (a) extracting Taheebo with an alcohol and concentrating an extract; and (b) lyophilizing the alcohol-extracted and concentrated Taheebo, and a composition for the treatment, prevention or amelioration of an inflammatory disease including a lyophilizate of a Taheebo alcohol extract prepared therefrom.

## Claims

### Claim 1

A method of preparing a lyophilizate of a Taheebo alcohol extract, comprising:
(a) extracting Taheebo with an alcohol and concentrating an extract; and
(b) lyophilizing the alcohol-extracted and concentrated Taheebo.

### Claim 2 (depends on 1)

The method of claim 1, wherein, in Step (a), a concentration of ethanol in the alcohol is in a range of 50 to 90 (v/v)%.

### Claim 3 (depends on 1)

The method of claim 1, wherein, in Step (a), the extracting is performed at a temperature of 40 to 80° C. for 3 to 10 hours.

### Claim 4 (depends on 1)

The method of claim 1, wherein, in Step (a), the lyophilizing is performed at a temperature of −30 to −50° C. for 48 to 168 hours.

### Claim 5 (depends on 1)

The method of claim 1, wherein the lyophilizate of a Taheebo alcohol extract has an inhibitory effect of nitric oxide synthesis.

### Claim 6 (depends on 1)

A composition for the prevention or treatment of an inflammatory disease, comprising a lyophilizate of a Taheebo alcohol extract prepared using the method according to claim 1.

### Claim 7

(canceled)

### Claim 8 (depends on 6)

The composition of claim 6, wherein said composition is a dietary supplement for the prevention or amelioration of an inflammatory disease.

### Claim 9 (depends on 6)

The composition of claim 6, wherein said composition is a skin preparation for external use for the prevention or treatment of an inflammatory disease.

### Claim 10 (depends on 6)

The composition of claim 6, wherein said composition is a cosmetic composition for the prevention or amelioration of an inflammatory disease.

### Claim 11 (depends on 6)

A method for preventing or treating an inflammatory disease in a subject, said method comprising administering a therapeutically effective amount of a composition of claim 6.

### Claim 12 (depends on 11)

The method of claim 11, wherein said inflammatory disease is selected from the group consisting of: allergy, dermatitis, atopy, conjunctivitis, periodontitis, rhinitis, otitis media, laryngopharyngitis, tonsillitis, pneumonia, gastric ulcer, gastritis, Crohn's disease, colitis, gout, ankylosing spondylitis, rheumatic fever, lupus, fibromyalgia, psoriatic arthritis, osteoarthritis, rheumatoid arthritis, periarthritis, tendonitis, tenosynovitis, peritendinitis, myositis, hepatitis, cystitis, nephritis, Sjogren's syndrome, multiple sclerosis, an acute inflammatory disease, a chronic inflammatory disease, and a combination thereof