# US 2019/0167734 A1 · Pharmaceutical composition and methods for the prevention and/or treatment of Staphylococcus aureus using artificial bacterial colonization

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- Number: US 2019/0167734 A1
- Document ID: US20190167734A1
- Status: Published application
- Kind: A1
- Application number: 16/270,296
- Filed: 2019-02-07
- Published: 2019-06-06
- Earliest claimed: 2017-08-08
- Assignees: Universite De Versailles-St Quentin En Yvelines; Assistance Publique-Hopitaux de Paris
- Applicants: Universite De Versailles-St Quentin En Yvelines; Assistance Publique - Hopitaux De Paris
- Inventors: Jean-Louis GAILLARD; Didier GUILLEMOT; Jean-Louis HERRMANN; Martin ROTTMAN; Anne-Sophie ALVAREZ; Jérôme SALOMON; Valérie LESELLIER
- Web page: https://patentgrep.com/patent/US20190167734A1

## CPC

- A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00)
- A61K 9/0014: {Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00)}
- A61K 9/0043: {Nose}
- A61K 9/19: lyophilised {, i.e. freeze-dried, solutions or dispersions (lyophilised products with subsequent particle size reduction A61K9/14; granules or pellets made by lyphilisation A61K9/1682; solid oral dosage forms made by lyophilisation A61K9/2095; lyophilisation additives A61K47/00)}
- A61K 35/00: Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K 35/66: Microorganisms or materials therefrom (fungi, yeasts or candida A61K36/06)
- A61P 31/04: Antibacterial agents
- C12N 1/20: Bacteria; Culture media therefor
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a pharmaceutical composition comprising at least one Corynebacterium sp, Staphylococcus pasteuri and, optionally, Staphyloccocus epidermidis for use as a medicament, in particular for use in the prevention or treatment of S. aureus colonization.

## Claims

### Claim at position 1

1-17. (canceled)

### Claim 18

A method for preventing and/or treating colonization by Staphylococcus aureus in a subject in need thereof, the method comprising administering a pharmaceutical composition comprising at least one Corynebacterium sp., Staphyloccocus pasteuri, and a pharmaceutically acceptable excipient.

### Claim 19 (depends on 18)

The method of claim 18, wherein said composition comprises at least one Corynebacterium sp. selected from the group consisting of C. accolens, C. propinquum, C. pseudodiphtheriticum, C. amycolatum, C. glutamicum, C. aurimucosum, C. tuberculostearicum, and C. afermentans.

### Claim 20 (depends on 18)

The method of claim 18, wherein said composition comprises at least 103 CFUs of Corynebacterium sp. and at least 103 CFUs of Staphyloccocus pasteuri.

### Claim 21 (depends on 18)

The method of claim 18, wherein said composition comprises a total of at least 103 bacterial CFUs per dose.

### Claim 22 (depends on 21)

The method of claim 21, wherein said composition comprises a total of at least 3×103 bacterial CFUs per dose.

### Claim 23 (depends on 18)

The method of claim 18, wherein said composition further comprises Staphylococcus epidermidis.

### Claim 24 (depends on 23)

The method of claim 23, wherein said composition comprises at least 103 CFUs of S. epidermidis per dose.

### Claim 25 (depends on 23)

The method of claim 23, wherein said composition comprises a ratio of Corynebacterium sp. to S. pasteuri to S. epidermidis in the range of 1:0.01:0.01 to 1:1:1.

### Claim 26 (depends on 18)

The method of claim 18, wherein said pharmaceutically acceptable excipient comprises at least one lyoprotectant.

### Claim 27 (depends on 26)

The method of claim 26, wherein said lyoprotectant is selected from the group consisting of peptone, glycerol, lactose, gelatin, glucose, sucrose, trehalose, dextran, maltodextrin, adonitol, and sodium glutamate.

### Claim 28 (depends on 18)

The method of claim 18, wherein said composition is lyophilized or freeze-dried, and is reconstituted with at least one pharmaceutically acceptable excipient prior to administration.

### Claim 29 (depends on 18)

The method of claim 18, wherein said composition further comprises at least one pharmaceutically acceptable gelling agent.

### Claim 30 (depends on 18)

The method of claim 18, wherein said composition is prepared as a patch, gel, cream, lotion, ointment, film, emulsion, or salve.

### Claim 31 (depends on 18)

The method of claim 18, comprising administration of the pharmaceutical composition to the anterior nares and/or the skin.

### Claim 32 (depends on 18)

The method of claim 18, wherein said S. aureus is methicillin-sensitive Staphylococcus aureus or methicillin-resistant Staphylococcus aureus.

### Claim 33

A pharmaceutical composition comprising at least one Corynebacterium sp., Staphyloccocus pasteuri, and a pharmaceutically acceptable excipient.

### Claim 34 (depends on 33)

The composition of claim 33, wherein said bacteria are lyophilized or freeze-dried.

### Claim 35 (depends on 33)

A kit comprising the pharmaceutical composition of claim 33, and:
a gel, a cream, a lotion, an ointment, an emulsion, or a salve, suitable for nasal use and a means for nasal administration, or
a gel, a cream, a lotion, an ointment, an emulsion, or a salve, suitable for skin use.