# US 2019/0167729 A1 · Oxygenated demineralized bone matrix for use in bone growth

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- Number: US 2019/0167729 A1
- Document ID: US20190167729A1
- Status: Published application
- Kind: A1
- Application number: 16/269,536
- Filed: 2019-02-06
- Published: 2019-06-06
- Earliest claimed: 2010-10-05
- Assignees: Theracell, Inc.; Yissum Research Development Company of the Hebrew University of Jerusalem
- Applicants: Theracell, Inc.; Yissum Research Development Company of the Hebrew University of Jerusalem, LTD.
- Inventors: Frank M. Phillips; Stephen H. Hochschuler; Dan Gazit; Gadi Pelled; Zulma Gazit
- Web page: https://patentgrep.com/patent/US20190167729A1

## CPC

- A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
- A61K 9/0026: {Blood substitute; Oxygen transporting formulations; Plasma extender}
- A61L 27/3608: {Bone, e.g. demineralised bone matrix [DBM], bone powder}
- A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease
- A61L 2430/02: for reconstruction of bones; weight-bearing implants
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

An improved composition for inducing bone growth is provided that is a combination of at least DBM and an oxygen carrier. Injection/implantation of a composition of DBM and an oxygen carrier (e.g. a perfluorocarbon) results in enhancement of bone formation compared to DBM alone.

## Claims

### Claim 1

A method of inducing bone growth, comprising:
implanting an effective amount of a composition for implantation within a patient for inducing bone growth at a target site in the patient, the composition consisting of:
a perfluorocarbon in an amount of about 5% to about 60% (w/v);
an emulsifier; and
dem ineralized bone matrix (DBM),
wherein the composition is free of cultured cells and is capable of inducing bone growth in the patient in the absence of cultured cells and the composition optionally consists of autologous bone chips, allograft bone chips, growth factors, fibrin, or collagen.

### Claim 2 (depends on 1)

The method of claim 1, wherein the perfluorocarbon is selected from the group consisting of perfluorobutylamine (PFTBA), perfluorooctylbromide (PFOB), perfluoro-n-octane, octafluoropropane, perfluorohexane, perfluorodecalin, perfluorodichlorooctane, perfluorodecane, perfluorotripropylamine, perfluorotrimethylcyclohexane, perfluoroperhydrophenanthrene, perfluoromethyladamantane, perfluorodimethyladamantane, perfluoromethyldecaline, perfluorofluorene, diphenyldimethylsiloxane, hydrogen-rich monohydroperfluorooctane, alumina-treated perfluorooctane, and mixtures thereof.

### Claim 3 (depends on 1)

The method of claim 1, wherein the perfluorocarbon is perfluorodecalin.

### Claim 4 (depends on 1)

The method of claim 1, wherein DBM is in the form of putty, gel, strips, paste, sheets, or fibers.

### Claim 5 (depends on 1)

The method of claim 1, wherein the DBM is in the form of fibers.

### Claim 6 (depends on 1)

The method of claim 1, where the emulsifier is lecithin.

### Claim 7

A method of inducing bone growth, consisting of:
implanting an effective amount of a composition for implantation within a patient for inducing bone growth at a target site in the patient, the composition consisting of:
a perfluorocarbon in an amount of about 5% to about 60% (w/v);
an emulsifier; and
dem ineralized bone matrix (DBM),
wherein the composition is free of cultured cells and is capable of inducing bone growth in the patient in the absence of cultured cells and the composition optionally consists of autologous bone chips, allograft bone chips, growth factors, fibrin, or collagen.

### Claim 8 (depends on 7)

The method of claim 7, wherein the perfluorocarbon is perfluorodecalin.

### Claim 9 (depends on 7)

The method of claim 7, wherein DBM is in the form of putty, gel, strips, paste, sheets, or fibers.

### Claim 10 (depends on 7)

The method of claim 7, wherein the DBM is in the form of fibers.

### Claim 11 (depends on 7)

The method of claim 7, where the emulsifier is lecithin.