# US 2019/0167708 A1 · Tumor suppressor tet1 and uses thereof

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- Number: US 2019/0167708 A1
- Document ID: US20190167708A1
- Status: Published application
- Kind: A1
- Application number: 15/988,619
- Filed: 2018-05-24
- Published: 2019-06-06
- Earliest claimed: 2017-05-26
- Assignees: The Chinese University of Hong Kong
- Applicants: The Chinese University of Hong Kong
- Inventors: Qian TAO; Lili LI
- Web page: https://patentgrep.com/patent/US20190167708A1

## CPC

- A61K 31/711: Natural deoxyribonucleic acids, i.e. containing only 2'-deoxyriboses attached to adenine, guanine, cytosine or thymine and having 3'-5' phosphodiester links
- A61K 31/713: Double-stranded nucleic acids or oligonucleotides
- A61K 48/0016: {wherein the nucleic acid is delivered as a 'naked' nucleic acid, i.e. not combined with an entity such as a cationic lipid}
- A61K 48/0058: {Nucleic acids adapted for tissue specific expression, e.g. having tissue specific promoters as part of a contruct}
- A61K 48/0066: {Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid}
- C12Q 1/6827: for detection of mutation or polymorphism
- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- C12Q 2600/154: Methylation markers
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention provides a method for assessing the risk of cancer in a subject by detecting elevated methylation level in the genomic sequence of the TET1 gene, which leads to suppressed expression of this gene. A kit and device useful for practicing such a method are also provided.

## Claims

### Claim at position 1

1-20. (canceled)

### Claim 21

A compound of Formula II-IX having the any one of the following structures:

### Claim 22 (depends on 21)

A pharmaceutical composition for producing a pluripotent stem cell from differentiated cells, the composition comprising the compound of claim 21 and a pharmaceutically acceptable salt.

### Claim 23

A mixture comprising differentiated cells and a compound of Formula II-IX having any one of the following structures:

### Claim 24 (depends on 23)

The mixture of claim 23, wherein the differentiated cells are a cellular aggregate.

### Claim 25 (depends on 21)

A method for producing a cellular aggregate from differentiated cells, wherein the cellular aggregate comprise a cell that expresses at least one pluripotent stem cell marker, the method comprising:
(a) culturing the differentiated cells at a density of about 1×105 to about 1×106 cells/ml in a vessel;
(b) mechanically agitating the differentiated cells to form the cellular aggregate;
(c) culturing the cellular aggregate under conditions to produce a cell that expresses at least one pluripotent stem cell marker; and
(d) exposing the cellular aggregate to an effective amount of the compound of claim 21.

### Claim 26 (depends on 25)

The method of claim 25, wherein the at least one pluripotent stem cell marker is Oct4, Sox2 or Nanog.

### Claim 27 (depends on 25)

The method of claim 25, further comprising subsequent to step (c) expanding the cell that expresses the at least one pluripotent stem cell marker.

### Claim 28 (depends on 25)

The method of claim 25, further comprising subsequent to step (c) isolating the cell that expresses the at least one pluripotent stem cell marker.