# US 2019/0166866 A1 · Method for producing a protein phospholipid complex from a crustacean catch

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- Number: US 2019/0166866 A1
- Document ID: US20190166866A1
- Status: Published application
- Kind: A1
- Application number: 16/206,600
- Filed: 2018-11-30
- Published: 2019-06-06
- Earliest claimed: 2017-12-04
- Assignees: Rimfrost Technologies AS
- Applicants: Rimfrost Technologies
- Inventors: Stig Remøy; Inge Bruheim
- Web page: https://patentgrep.com/patent/US20190166866A1

## CPC

- A23D 9/007: characterised by ingredients other than fatty acid triglycerides
- A23J 1/04: from fish or other sea animals
- A23J 3/04: Animal proteins
- A23J 3/341: {of animal proteins}
- A23L 17/40: Shell-fish
- A23L 33/115: Fatty acids or derivatives thereof; Fats or oils
- A23L 33/18: Peptides; Protein hydrolysates
- A61K 35/60: Fish, e.g. seahorses; Fish eggs
- C11B 1/10: by extracting
- A23D: EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS (preservation thereof A23B20/00; production, refinement or preservation of animal or vegetable fats or oils C11B, C11C)
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C11B: PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES (drying-oils C09F)

## Abstract

A method of producing a protein phospholipid complex from a crustacean catch including protein is claimed where hydrolysis occurs without substantially denaturing the protein from the crustacean catch. A method of producing a stable protein phospholipid emulsion from a crustacean catch is also claimed. A method of producing crustacean oil is claimed where the oil is separated from the hydrolyzed crustacean catch. Other methods of producing a protein phospholipid complex are claimed which relate to not removing the shell, removing the shell, and removing then adding back the shell to form the protein phospholipid complex.

## Claims

### Claim 1

A method of producing a protein phospholipid complex comprising the steps of
a) disintegrating a crustacean catch to provide a disintegrated crustacean catch comprising protein;
b) contacting the disintegrated crustacean catch with a proteolytic enzyme to provide a hydrolyzed crustacean catch without substantially denaturing the protein; and
c) separating the hydrolyzed crustacean catch to provide a protein phospholipid complex.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said contacting further includes a second enzyme.

### Claim 3 (depends on 2)

The method according to claim 2, wherein said second enzyme comprises a chitinase, a collagenase, or another proteolytic enzyme.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the proteolytic enzyme is a mixture of acid, neutral, and alkaline proteases.

### Claim 5 (depends on 1)

The method according to claim 1, wherein the proteolytic enzyme comprises a first protease mixture and a second protease mixture.

### Claim 6 (depends on 5)

The method according to claim 5, wherein the first protease mixture comprises at least one alkaline protease; and the second protease mixture comprises an acid protease, a neutral protease, and an alkaline protease.

### Claim 7 (depends on 5)

The method according to claim 5, wherein the first protease mixture comprises 0.3-0.5% of the total weight of the disintegrated crustacean catch; and the second protease mixture comprises 0.03-0.05% of the total weight of the disintegrated crustacean catch.

### Claim 8 (depends on 5)

The method according to claim 5, wherein the proteases are from Bacillus Licheniformis.

### Claim 9 (depends on 1)

The method according to claim 1, wherein the contacting of step (b) includes contacting with a cell wall degrading enzyme.

### Claim 10 (depends on 1)

The method according to claim 1, wherein the disintegrated crustacean catch is contacted with a proteolytic enzyme for over 100 minutes to provide a hydrolyzed crustacean catch.

### Claim 11 (depends on 1)

The method according to claim 1, wherein said crustacean catch comprises krill.

### Claim 12 (depends on 1)

The method according to claim 1, wherein said disintegrating comprises one or more of pulping, milling, grinding or shredding.

### Claim 13 (depends on 1)

The method according to claim 1, wherein said contacting comprises an organic solvent.

### Claim 14 (depends on 1)

The method according to claim 1, wherein said contacting comprises incubating the disintegrated crustacean catch at a temperature range of 45-75° C.

### Claim 15 (depends on 1)

The method according to claim 1, wherein said protein phospholipid complex has a water content less than 15% w/w.

### Claim 16 (depends on 1)

The method according to claim 1, wherein the crustacean catch protein comprise digestive enzymes and other protein.

### Claim 17

A method of producing a stable protein phospholipid emulsion comprising the steps of
a) disintegrating a crustacean catch to provide a disintegrated crustacean catch;
b) contacting the disintegrated crustacean catch with a proteolytic enzyme to provide a hydrolyzed crustacean catch; and
c) separating the hydrolyzed crustacean catch to provide a stable protein phospholipid emulsion.

### Claim 18 (depends on 17)

The method according to claim 17, wherein said contacting further includes a second enzyme.

### Claim 19 (depends on 18)

The method according to claim 18, wherein said second enzyme comprises a chitinase, a collagenase, or another proteolytic enzyme.

### Claim 20 (depends on 17)

The method according to claim 17, wherein the proteolytic enzyme is a mixture of acid, neutral, and alkaline proteases.

### Claim 21 (depends on 17)

The method according to claim 17, wherein said crustacean catch comprises krill.

### Claim 22 (depends on 17)

The method according to claim 17, wherein said disintegrating comprises one or more of pulping, milling, grinding or shredding.

### Claim 23 (depends on 17)

The method according to claim 17, wherein said contacting comprises an organic solvent.

### Claim 24 (depends on 17)

The method according to claim 17, wherein said contacting comprises incubating the disintegrated crustacean catch at a temperature range of 45-75° C.

### Claim 25 (depends on 17)

The method according to claim 17, wherein said stable protein phospholipid emulsion comprises 45-55% water content v/v.

### Claim 26

A stable protein phospholipid emulsion comprising: water, protein, and lipid.

### Claim 27

A method of producing crustacean oil comprising the steps of
a) disintegrating a crustacean catch to provide a disintegrated crustacean catch comprising protein;
b) contacting the disintegrated crustacean catch with one or more proteolytic enzyme to provide a hydrolyzed crustacean catch without substantially denaturing the protein; and
c) separating the hydrolyzed crustacean catch to provide a crustacean oil.

### Claim 28 (depends on 27)

The method according to claim 27 wherein the crustacean oil is hill oil and the disintegrated crustacean catch is disintegrated krill.

### Claim 29 (depends on 27)

The method according to claim 27, wherein the proteolytic enzymes comprise a mixture of acid, neutral, and alkaline proteases.

### Claim 30 (depends on 27)

The method according to claim 27, wherein the method is performed in the absence of extraction.

### Claim 31

A nutritional supplement comprising a crustacean oil and another oil selected from the group consisting of flaxseed oil, pumpkinseed oil, canola oil, soybean oil, walnut oil, fish oil, seal oil, microalgae oil, mussels oil, and shrimp oil.

### Claim 32 (depends on 31)

A nutritional supplement according to claim 31, wherein the another oil is green-lipped mussel oil.

### Claim 33 (depends on 31)

A nutritional supplement according to claim 31, wherein the crustacean oil is krill oil.

### Claim 34

A method of producing a protein phospholipid complex comprising the steps of
a) disintegrating a crustacean catch to provide a disintegrated crustacean catch comprising protein;
b) contacting the disintegrated crustacean catch with a proteolytic enzyme to provide a hydrolyzed crustacean catch without denaturing the protein; and
c) separating the hydrolyzed crustacean catch to provide a protein phospholipid complex, wherein the shell is not removed from the hydrolyzed crustacean catch during the separating step and is part of the protein phospholipid complex.

### Claim 35 (depends on 34)

The method according to claim 34, wherein the crustacean catch is hill.

### Claim 36

A method of producing a protein phospholipid complex comprising the steps of
a) disintegrating a crustacean catch to provide a disintegrated crustacean catch comprising protein;
b) contacting the disintegrated crustacean catch with a proteolytic enzyme to provide a hydrolyzed crustacean catch without denaturing the protein; and
c) separating the hydrolyzed crustacean catch to provide a protein phospholipid complex comprising the steps of (1) separating shells from the hydrolyzed crustacean catch; (2) pasteurizing the hydrolyzed crustacean catch to inactivate the enzyme; (3) removing excess water by separation; and (4) adding shells back to the hydrolyzed crustacean catch to provide a protein phospholipid complex.

### Claim 37 (depends on 36)

The method according to claim 36, wherein the shells are separated from the hydrolyzed crustacean catch in step (1) with a filter or sieve.

### Claim 38 (depends on 36)

The method according to claim 36, wherein the excess water is removed in step (3) by horizontal centrifuge.

### Claim 39 (depends on 36)

The method according to claim 36, wherein the shells are added back in step (4) along with an insoluble particle for purposes of utility.