# US 2019/0166849 A1 · Bacillus methylotrophicus strain dr-08 producing natural volatile compound and having antibacterial activity, and use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0166849 A1 - Document ID: US20190166849A1 - Status: Published application - Kind: A1 - Application number: 16/320,797 - Filed: 2017-07-25 - Published: 2019-06-06 - Earliest claimed: 2016-07-28 - Assignees: Global Agro Co., Ltd. - Applicants: Global Agro Co., Ltd. - Inventors: Jin-Cheol KIM; Nan Hee YU; Hee Won JEON; Seong Mi IM; Ki Hyun KIM - Web page: https://patentgrep.com/patent/US20190166849A1 ## CPC - A01N 63/22: Bacillus - A01C 1/06: Coating or dressing seed - A01C 1/08: Immunising seed - A01N 25/14: wettable - A01N 27/00: Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons - A01N 31/02: Acyclic compounds - A01N 35/02: containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals - A01N 37/02: Saturated carboxylic acids or thio analogues thereof; Derivatives thereof - C12N 1/20: Bacteria; Culture media therefor - C12N 1/205: Bacterial isolates - C12R 2001/07: Bacillus - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - A01C: PLANTING; SOWING; FERTILISING (parts, details or accessories of agricultural machines or implements, in general A01B51/00 - A01B75/00) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS ## Abstract The present invention relates to Bacillus methylotrophicus strain DR-08 which produces a natural volatile compound and has antimicrobial activity, and a use thereof. The Bacillus methylotrophicus strain DR-08 according to the present invention, which is an antagonistic microorganism for controlling phytopathogenic microorganisms, and secondary metabolites produced by the strain, which include antibacterially- and antifungally-active non-volatile and volatile materials, are harmless to humans and domestic animals, and exhibit excellent control activity against various phytopathogenic bacteria and fungi while not causing environmental pollution. Thus, the strain and the secondary metabolites can be very usefully used for controlling various plant diseases in an environmentally-friendly manner. ## Claims ### Claim 1 Bacillus methylotrophicus strain DR-08 (accession no. KCTC13060BP) which produces a natural volatile compound and has antimicrobial activity against phytopathogenic bacteria and fungi. ### Claim 2 (depends on 1) The strain of claim 1, wherein the natural volatile compound is one or more selected from the group consisting of 2,3,4-trimethylpentane, 2,3,3-trimethylpentane, 2,3-dimethylhexane, 3-methyl-1-phenyl-2-butanone, 2-methylpropanoic acid, 2,2,5-trimethylhexane, 2,2,3-trimethylhexane, 4,4-dimethylpent-2-yl ester formic acid, 2,4-dimethyl-1-heptane, 3-methylbutanoic acid, and 2-methylbutanoic acid. ### Claim 3 (depends on 1) The strain of claim 1, wherein the phytopathogenic bacteria is one or more strains selected from the group consisting of strains Acidovorax avenae subsp. cattleyae, Agrobacterium tumefaciens, Burkholderia glumae, Clavibacter michiganensis subsp. michiganensis, Pectobacterium carotovora subsp. carotovora, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. lachrymans, Xanthomonas arboricola pv. pruni, Xanthomonas campestris pv. citri, Xanthomonas euvesicatoria, Xanthomonas oryzae pv. oryzae, and Ralstonia solanacearum. ### Claim 4 (depends on 1) The strain of claim 1, wherein the phytopathogenic fungi is one or more strains selected from the group consisting of strains Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium graminearum, Fusarium oxysporum f. sp. lycopersici, Fusarium verticillioides, Magnaporthe oryzae, Phytophthora capsici, Rhizoctonia solani, and Raffaelea quercus-mongolicae. ### Claim 5 (depends on 1) A plant disease-controlling composition, comprising as an active ingredient: one or more selected from the group consisting of the strain according to claim 1, a natural volatile compound produced from the strain, a culture of the strain, a concentrate of the culture, a dried product of the culture, and an extract of the culture. ### Claim 6 (depends on 5) The plant disease-controlling composition of claim 5, wherein the extract is a butanol extract. ### Claim 7 (depends on 5) The plant disease-controlling composition of claim 5, wherein the plant disease-controlling composition has a wettable powder formulation. ### Claim 8 (depends on 5) The plant disease-controlling composition of claim 5, wherein the plant disease is a plant disease which develops due to any one phytopathogenic bacteria selected from the group consisting of strains Acidovorax avenae subsp. cattleyae, Agrobacterium tumefaciens, Burkholderia glumae, Clavibacter michiganensis subsp. michiganensis, Pectobacterium carotovora subsp. carotovora, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. lachrymans, Xanthomonas arboricola pv. pruni, Xanthomonas campestris pv. citri, Xanthomonas euvesicatoria, Xanthomonas oryzae pv. oryzae, and Ralstonia solanacearum, or develops due to any one phytopathogenic fungi selected from the group consisting of strains Botrytis cinerea, Colletotrichum coccodes, Endothia parasitica, Fusarium graminearum, Fusarium oxysporum f. sp. lycopersici, Fusarium verticillioides, Magnaporthe oryzae, Phytophthora capsici, Rhizoctonia solani, and Raffaelea quercus-mongolicae. ### Claim 9 (depends on 5) A method for controlling a plant disease, comprising: a step of applying an effective amount of the plant disease-controlling composition according to claim 5 to a plant part, soil for cultivating the plant, or a seed. ### Claim 10 (depends on 1) A method for producing a plant disease-controlling composition, comprising: a step of culturing the Bacillus methylotrophicus strain DR-08 according to claim 1. ### Claim 11 (depends on 1) An antimicrobial composition against phytopathogenic bacteria or fungi, comprising as an active ingredient: iturin A, oxydifficidin, or difficidin which is isolated from the strain according to claim 1.