# US 2019/0166781 A1 · Lettuce variety 45-100 rz

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- Number: US 2019/0166781 A1
- Document ID: US20190166781A1
- Status: Published application
- Kind: A1
- Application number: 16/168,930
- Filed: 2018-10-24
- Published: 2019-06-06
- Earliest claimed: 2017-10-24
- Assignees: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Applicants: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Inventors: Maria Helena Roosenboom-Kooijmans; Willem van Vliet
- Web page: https://patentgrep.com/patent/US20190166781A1

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/12: Leaves
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

The present invention relates to a Lactuca sativa seed designated 45-100 RZ. The present invention also relates to a Lactuca sativa plant produced by growing the 45-100 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 45-100 RZ.

## Claims

### Claim 1

A seed of lettuce variety 45-100 RZ, a sample of seed of said variety having been deposited under NCIMB accession No. 42847.

### Claim 2 (depends on 1)

A lettuce plant grown from the seed of claim 1.

### Claim 3 (depends on 2)

The lettuce plant of claim 2, which is a plant grown from seed having been deposited under NCIMB accession No. 42847.

### Claim 4 (depends on 2)

A lettuce plant, or a part thereof, having all the physiological and morphological characteristics of the lettuce plant of claim 2.

### Claim 5 (depends on 2)

A part of the plant of claim 2, wherein said part comprises a microspore, pollen, ovary, ovule, embryo sac, egg cell, cutting, root, stem, cell or protoplast.

### Claim 6 (depends on 2)

A tissue culture of regenerable cells or protoplasts from the lettuce plant of claim 2.

### Claim 7 (depends on 6)

The tissue culture as claimed in claim 6, wherein said cells or protoplasts of the tissue culture are derived from a tissue comprising a leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, anther, flower, seed or stem.

### Claim 8 (depends on 6)

A lettuce plant regenerated from the tissue culture of claim 6, wherein the regenerated plant expresses all of the physiological and morphological characteristics of lettuce variety 45-100 RZ, a sample of seed of said variety having been deposited under NCIMB accession No. 42847.

### Claim 9

A method of vegetatively propagating a plant of lettuce variety 45-100 RZ comprising (a) collecting tissue capable of being propagated from a plant of lettuce 45-100 RZ, a sample of seed of said variety having been deposited under NCIMB accession No. 42847, (b) cultivating the tissue to obtain proliferated shoots and rooting the proliferated shoots to obtain rooted plantlets, and (c) optionally growing plants from the rooted plantlets.

### Claim 10

A method for producing a progeny plant of lettuce cultivar 45-100 RZ, comprising crossing a plant designated 45-100 RZ with itself or with another lettuce plant, harvesting the resultant seed, and growing said seed.

### Claim 11 (depends on 10)

A progeny plant produced by the method of claim 10, wherein said progeny plant exhibits a combination of traits including resistance to lettuce aphid Nasonovia ribisnigri (Mosley) biotype Nr:0, resistance to root aphid (Pemphigus bursarius), resistance to downy mildew (Bremia lactucae) races B1:1-33EU and Ca-I-VIII, mature medium to grey green leaves with slight to no blistering and a medium size head.

### Claim 12 (depends on 10)

A progeny plant produced by the method of claim 10, wherein said progeny exhibits all the morphological and physiological characteristics of the lettuce variety designated 45-100 RZ, a sample of seed of said variety having been deposited under NCIMB accession No. 42847.

### Claim 13 (depends on 11)

The progeny plant as claimed in claim 11, wherein said progeny plant is modified in one or more other characteristics.

### Claim 14 (depends on 13)

The progeny plant as claimed in claim 13, wherein the modification is effected by mutagenesis.

### Claim 15 (depends on 13)

The progeny plant as claimed in claim 13, wherein the modification is effected by transformation with a transgene.

### Claim 16 (depends on 2)

A method of producing a lettuce seed comprising crossing a male parent lettuce plant with a female parent lettuce plant and harvesting the resultant lettuce seed, wherein said male parent lettuce plant or said female parent lettuce plant is the lettuce plant of claim 2.

### Claim 17 (depends on 16)

A lettuce seed produced by the method of claim 16.

### Claim 18 (depends on 17)

A lettuce plant produced by growing the seed of claim 17.

### Claim 19

A method for producing a seed of a 45-100 RZ-derived lettuce plant comprising (a) crossing a plant of lettuce variety 45-100 RZ, a sample of seed of which having been deposited under NCIMB Accession No. NCIMB 42847, with a second lettuce plant, and (b) whereby seed of a 45-100 RZ-derived lettuce plant forms.

### Claim 20 (depends on 19)

The method of claim 19 further comprising (c) crossing a plant grown from 45-100 RZ-derived lettuce seed with itself or with a second lettuce plant to yield additional 45-100 RZ-derived lettuce seed, (d) growing the additional 45-100 RZ-derived lettuce seed of step (c) to yield additional 45-100 RZ-derived lettuce plants, and (e) repeating the crossing and growing of steps (c) and (d) for an additional 3-10 generations to generate further 45-100 RZ-derived lettuce plants, and (f) whereby seed of a 45-100 RZ-derived lettuce plant forms.

### Claim 21 (depends on 19)

A seed produced by the method of claim 19.

### Claim 22 (depends on 21)

A plant grown from the seed of claim 21.

### Claim 23

A method of introducing at least one new trait into a plant of lettuce variety 45-100 RZ comprising: (a) crossing a plant of lettuce variety 45-100 RZ, a sample of seed of which having been deposited under NCIMB Accession No. NCIMB 42847, with a second lettuce plant that comprises at least one new trait to produce progeny seed, (b) harvesting and planting the progeny seed to produce at least one progeny plant of a subsequent generation, wherein the progeny plant comprises the at least one new trait, (c) crossing the progeny plant with a plant of lettuce variety 45-100 RZ to produce backcross progeny seed, (d) harvesting and planting the backcross progeny seed to produce a backcross progeny plant, and (e) repeating steps (c) and (d) for at least three additional generations to produce a lettuce plant of variety 45-100 RZ comprising at least one new trait and all of the physiological and morphological characteristics of a plant of lettuce variety 45-100 RZ, when grown in the same environmental conditions.

### Claim 24

A method of producing a plant of lettuce variety 45-100 RZ comprising at least one new trait, the method comprising introducing a mutation or transgene conferring the at least one new trait into a plant of lettuce variety 45-100 RZ, wherein a sample of seed of said variety has been deposited under NCIMB Accession No. NCIMB 42847.

### Claim 25 (depends on 23)

The lettuce plant produced by the method of claim 23.

### Claim 26 (depends on 1)

A method for producing lettuce leaves as a food product comprising sowing the seed of a claim 1 and growing the seed into a harvestable lettuce plant and harvesting the head or leaves of said plant.

### Claim 27 (depends on 2)

A method for producing lettuce leaves as a fresh vegetable comprising packaging leaves of a plant of claim 2.

### Claim 28 (depends on 2)

A method for producing lettuce leaves as a processed food comprising processing leaves of a plant of claim 2.

### Claim 29 (depends on 2)

A container comprising one or more lettuce plants of claim 2 for harvest of leaves.

### Claim 30 (depends on 2)

A method of determining the genotype of a plant of lettuce variety 45-100 RZ, a sample of seed of which has been deposited under NCIMB Accession No. NCIMB 42847, or a first generation progeny thereof, comprising obtaining a sample of nucleic acids from said plant and comparing said nucleic acids to a sample of nucleic acids obtained from a reference plant, and detecting a plurality of polymorphisms between the two nucleic acid samples, wherein the plurality of polymorphisms are indicative of lettuce variety 45-100 RZ and/or give rise to the expression of any one or more, or all, of the physiological and morphological characteristics of lettuce variety 45-100 RZ as claimed in claim 2.

### Claim 31 (depends on 24)

A lettuce plant produced by the method of claim 24.