Method, composition and sensor for analyte detection
AssigneesCambridge Display Technology Limited, Sumitomo Chemical Company, Limited
InventorJonathan Behrendt
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Abstract
A method of testing a liquid sample for the presence of an analyte, the method comprising the steps of: forming a mixture by contacting the sample with a composition comprising an oxidase for formation of hydrogen peroxide from the analyte, a fluorescent indicator precursor capable of forming a fluorescent indicator in the presence of an oxygen radical and an iron compound wherein the iron compound is dissolved in the mixture; irradiating the mixture; and measuring fluorescence from the fluorescent indicator. The method may be carried out using a device in which the mixture in a channel or chamber (101) of a microfluidic device is irradiated by light from light source (103) and emission from the fluorescent indicator is detected by photodetector (105).
Claims22
A method of testing a liquid sample for the presence of an analyte, the method comprising the steps of:
- forming a mixture by contacting the sample with a composition comprising an oxidase for formation of hydrogen peroxide from the analyte, a fluorescent indicator precursor capable of forming a fluorescent indicator in the presence of an oxygen radical and an iron compound wherein the iron compound is dissolved in the mixture;
- irradiating the mixture;
- and
- measuring fluorescence from the fluorescent indicator.
(canceled)
A composition comprising an oxidase for formation of hydrogen peroxide from an analyte;
- an iron compound;
- and a fluorescent indicator precursor capable of forming a fluorescent indicator in the presence of an oxygen radical, wherein the fluorescent indicator precursor is selected from the group consisting of:
- fluoresceins, rhodamines, coumarins, boron-dipyrromethenes, naphthalimides, perylenes, benzanthrones, benzoxanthrones;
- and benzothiooxanthrones.
(canceled)
Dependencies are inferred from the claim text.