Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsSean C. Bendall, Garry P. Nolan, Robert M. Angelo
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Abstract
A method of generating a high resolution two-dimensional image of a sample comprising cells and extracellular structures is provided. In certain embodiments, the method comprises: labeling a sample with at least one mass tag, thereby producing a labeled sample; scanning the sample with a secondary ion mass spectrometer (SIMS) ion beam to generate a data set that comprises spatially-addressable measurements of the abundance of the mass tag across an area of the sample; and outputting the data set. In many embodiments, the data set contains the identity and abundance of the mass tag. A system for performing the method is also provided.
Claims2
A method of generating a high resolution two-dimensional image of a sample comprising cells and extracellular structures, the method comprising:
- labeling a sample with at least one mass tag, thereby producing a labeled sample in which a biological feature of interest is bound to said at least one mass tag;
- scanning the sample with a secondary ion mass spectrometer (SIMS) ion beam to generate a data set that comprises spatially-addressable measurements of the abundance of said at least one mass tag across an area of said sample;
- and
- outputting the data set.
-24. (canceled)
Dependencies are inferred from the claim text.