Analysis method of protein-protein interacton and a screening method of protein-protein interaction inhibitors using a nanopore
AssigneesKorea Research Institute of Bioscience and Biotech Nology, Seoul National University R&Db Foundation
InventorsSeung Wook Chi, Ki Bum Kim, Dong Kyu Kwak, Mi Kyung Lee, Hong Sik Chae, Ji Hyang Ha
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Abstract
The present invention relates to a method for screening protein-protein interaction inhibitors using a nanopore, a method for analyzing protein structures, a method for analyzing protein-protein interactions, and a kit therefor.
Claims20
A method for screening protein-protein interaction inhibitors using a nanopore, comprising:
- (a) forming a protein complex in which a first protein and a second protein are bound by a protein-protein interaction;
- (b) measuring the intensity of an electrical signal generated while the protein complex passes through the nanopore;
- and
- (c) treating the protein complex with a protein-protein interaction inhibitor candidate, and measuring the intensity of an electrical signal generated while the protein complex passes through the nanopore.
A method for analyzing the structure of an interacting protein using a nanopore, comprising measuring the electrical signal generated while a free protein or protein complex passes through the nanopore.
A method for analyzing a protein-protein interaction using a nanopore, comprising:
- (a) passing a first protein or second protein through a nanopore;
- and measuring the electrical signal generated thereby;
- (b) forming a protein complex in which the first protein and the second protein are bound by a protein-protein interaction;
- and measuring the electrical signal generated while the protein complex passes through the nanopore;
- and
- (c) comparing the electrical signals generated in (a) and (b).
Dependencies are inferred from the claim text.