Interdigitated electrodes for in vitro analysis of cells
AssigneeUniversity of Central Florida Research Foundation, Inc.
InventorsSwaminathan RAJARAMAN, Jayan THOMAS
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Abstract
Described are interdigitated electrodes, which may optionally be plasmonic, useful for in vitro biosensing applications. Such devices may significantly reduce undesired background noise by separating the excitation source (light) from the detection signal (current), and thereby, leading to higher sensitivity for bioanalysis compared with conventional interdigitated electrodes. Also described are methods of making such interdigitated electrodes, which allow a substrate, which may optionally be plasmonic, to be tuned not only to maximize the targeted interaction of the cells with the nanoscale geometry, but also for the excitation wavelength to minimize biological sample interference.
Claims21
An interdigitated electrode device comprising interdigitated electrodes disposed on a nanostructured polymer layer.
A method of making an interdigitated electrode device comprising interdigitated electrodes associated with a nanostructured polymer layer, the method comprising:
- fabricating a nanostructured polymer layer;
- immobilizing the nanostructured polymer layer on a substrate;
- aligning a shadow mask on the nanostructured polymer layer;
- and
- depositing a conductive material onto the nanostructured polymer layer to form the interdigitated electrode device comprising interdigitated electrodes associated with the nanostructured polymer layer.
A method of monitoring a plurality of cells, the method comprising:
- disposing the plurality of cells on interdigitated electrodes associated with a nanostructured polymer layer;
- and
- irradiating the nanostructured polymer layer with a light source;
- and
- measuring impedance across the interdigitated electrodes.
Dependencies are inferred from the claim text.