Biological Material Quantifying Method, Image Processing Device, Pathological Diagnosis Support System and Recording Medium

An image processing device that quantifies a specific biological material in a specimen that has been stained using fluorescent dye accumulating particles capable of binding to the specific biological material, the image processing device comprising:

• an input unit that inputs a first fluorescence image obtained by capturing an image of the specimen;
• and
• a hardware processor that extracts a certain region from the first fluorescence image, calculates a first luminance integrated value by integrating luminance values of the certain region, and
• calculates the number of the fluorescent dye accumulating particles included in the certain region from the first luminance integrated value and an average luminance value per said fluorescent dye accumulating particle,
• wherein the average luminance value is calculated from a distribution of second luminance integrated values obtained by integrating luminance values for individual bright spot regions, which indicate emission of light by the fluorescent dye accumulating particles, in a second fluorescence image obtained by capturing an image of a preparation onto which the fluorescent dye accumulating particles are dispersed without agglomerating.

A non-temporary, computer-readable recording medium that stores a program, the program causing a computer that quantifies a specific biological material in a specimen that has been stained using fluorescent dye accumulating particles capable of binding to the specific biological material to:

• extract a certain region from a first fluorescence image obtained by capturing an image of the specimen;
• calculate a first luminance integrated value by integrating luminance values of the certain region;
• and
• calculate the number of the fluorescent dye accumulating particles included in the certain region from the first luminance integrated value and an average luminance value per said fluorescent dye accumulating particle,
• wherein the average luminance value is calculated from a distribution of second luminance integrated values obtained by integrating luminance values for individual bright spot regions, which indicate emission of light by the fluorescent dye accumulating particles, in a second fluorescence image obtained by capturing an image of a preparation onto which the fluorescent dye accumulating particles are dispersed without agglomerating.