Analysis method and system for analyzing a nucleic acid amplification reaction

Abstract

An analysis method is provided aimed at improving the linearity (precision and/or accuracy) of a quantification by a real-time nucleic acid amplification reaction RNR such as a polymerase chain reaction PCR over a wide range of analyte concentrations and/or limiting the effects of the presence of interfering substances by way of determining a quantification cycle number (Cq) of the RNR as the cycle number corresponding to an intersection of the growth curve with a combined threshold function (CTF) over the RNR cycle range comprising at least two different threshold levels, the quantification cycle number (Cq) being indicative of a quantitative and/or qualitative analysis result of a growth curve, the growth curve being indicative of the intensity of the fluorescence emission of an analyte for each amplification reaction cycle of the RNR over a RNR cycle range.

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