Combined optical-spectroscopic method for identifying microbial pathogens
AssigneesLeibniz-Institut Fur Photonische Technologien E.V., UniversitäTsklinikumjena
InventorsUte Neugebauer, Jürgen POPP, Ondrej STRANIK, Bettina LÖFFLER
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Abstract
Exemplary methods can be provided for the determination of a microbial pathogen. In addition, exemplary methods can be provided for the determination of a microbial pathogen and its anti-infective resistance. Further, an exemplary method can be provided for determining a bacterium and its antibiotic resistance. Systems and computer-accessible media can be provided for the determination of a microbial pathogen and its anti-infective resistance.
Claims21
1-15. (canceled)
A method for determining a microbial pathogen and an anti-infective resistance thereof in a sample comprising the microbial pathogen and an anti-infective, the method comprising:
- cultivating the sample comprising at least one microbial pathogen and an anti-infective agent;
- determining a Raman spectrum of the microbial pathogen by a Raman spectroscopy of the sample under at least two different anti-infective conditions;
- determining a morphology of the microbial pathogen by an optical detection of the sample under the at least two different anti-infective conditions;
- determining the microbial pathogen based on the Raman spectrum and the morphology of the microbial pathogen, and
- determining the anti-infective resistance of the microbial pathogen based on the Raman spectrum and the morphology of the microbial pathogen.
A system for determining of a microbial pathogen and an anti-infective resistance thereof, comprising:
- a Raman spectrometer configured to provide a Raman spectrum of the microbial pathogen;
- a device configured to provide an optical detection of the microbial pathogen so as to provide optical data of the microbial pathogen, wherein the system includes a computer processor that is configured to determine the morphology of the microbial pathogen by analyzing the optical data and determine the microbial pathogen based on a combination of the Raman spectrum and the morphology of the microbial pathogen, and
- wherein the computer process is further configured to determine the anti-infective resistance based on a combination of the Raman spectrum and the morphology of the microbial pathogen.
A computer-accessible media including a computer program element thereon that, when executed on a computer processor, configures the computer processor to perform procedures comprising:
- determining a morphology of a microbial pathogen based on data of a device for an optical detection of the pathogen;
- and
- determining the microbial pathogen based on a Raman spectrum of the microbial pathogen and the morphology of the microbial pathogen;
- and
- determining an anti-infective resistance of the microbial pathogen based on the Raman spectrum and the morphology of the microbial pathogen.
Dependencies are inferred from the claim text.