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AssigneesBeijing Institute of Genomics, Chinese Academy of Sciences, Peking University Third Hospital, The Third Affiliated Hospital of Guangzhou Medical University
InventorsJiang Liu, Jie Qiao, Guoqiang Li, Yang Yu, Yong Fan, Congru Li
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Disclosed in the present invention is a non-invasive detection method for screening for a well-developed blastocyst. A DNA methylation profile of a few of trophoblastic cells in a blastocyst is detected, and the average methylation level and the methylation pattern of embryos identified as having a good morphology in Gardner morphological blastocyst grading process are used as the standard for screening for well-developed blastocysts. The nearer the methylation level of the trophoblastic cells of the blastocyst to be tested approaches the methylation level or state of the good embryo, the better the embryo development is and the higher the suitability for being implanted into a maternal subject is. Furthermore, results of methylation sequencing may be analyzed to determine whether a chromosome is abnormal, so as to directly exclude chromosome-abnormal embryos.
A non-invasive detection method for screening for a well-developed blastocyst, characterized in that, genome of trophoblastic cells of the blastocyst to be detected is subjected to methylation detection;
A method for increasing the birth rate of test-tube baby, characterized in that, a blastocyst having a DNA methylation level of trophoblastic cells close or equal to the DNA methylation level and/or DNA methylation pattern of embryos identified as having a good morphology in Gardner morphological blastocyst grading system is selected for transplantation, and wherein the embryos identified as having a good morphology in the Gardner morphological blastocyst grading system refer to blastocysts of AA, AB, BA and BB grades.
A kit for screening for a well-developed blastocyst, characterized in that, the kit is for methylation sequencing of blastocysts, the DNA methylation level of embryos identified as having a good morphology in Gardner morphological blastocyst grading system is used as a standard, and a blastocyst to be detected is well-developed when its DNA methylation level is close or equal to the standard, and wherein the embryos identified as having a good morphology in the Gardner morphological blastocyst grading system refer to blastocysts of AA, AB, BA and BB grades.
(canceled)
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