Marker and Method for Determining the Composition or Purity of a Cell Culture and for Determining In Vitro the Identity of a Cartilage Cell or Synovial Cell
AssigneeTETEC Tissue Engineering Technologies AG
InventorsKarin Benz, Christoph Gaissmaier
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Abstract
The use of a marker for determining the composition or purity of a cell culture, wherein the marker is at least one marker selected from the group consisting of MMP1, ACE, POSTN, SYNPO, SULF1, ARH-GEF28, IGF2BP1, BAIAP2L1, LIMCH1, SCIN, DCLK1, PPL, CRABP2, NES, XPNPEP2 and SLIT3, and the expression level of the at least one marker is determined.
Claims15
-15. (canceled)
A method for assessing the composition or purity of a cell culture, comprising the following steps:
- a) obtaining a cell sample from a cell culture,
- b) determining the expression level of at least one marker in the cell sample and
- c) determining the composition or purity of the cell culture based on the expression level of the at least one marker,
- characterized in that the at least one marker is selected from the group consisting of ACE, MMP1, POSTN, SYNPO, SULF1, ARHGEF28, IGF2BP1, BAIAP2L1, LIMCH1, SCIN, DCLK1, PPL, CRABP2, NES, XPNPEP2, SLIT3 and combinations of at least two of the aforementioned markers.
A method for producing a medicinal product for novel therapies, more particularly a tissue engineering product, comprising the step of using a cell culture, wherein the composition or purity of the cell culture is determined by means of at least one marker selected from the group consisting of ACE, MMP1, POSTN, SYNPO, SULF1, ARHGEF28, IGF2BP1, BAIAP2L1, LIMCH1, SCIN, DCLK1, PPL, CRABP2, NES, XPNPEP2 and SLIT3.
Dependencies are inferred from the claim text.