Photocleavable nucleotide reagents with high stability
AssigneeRoche Molecular Systems, Inc.
InventorsAlexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
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Abstract
The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
Claims10
A method of detecting the presence or absence of a target nucleic acid sequence in a sample comprising:
- a) performing an amplifying step comprising contacting the sample with amplification reagents to produce an amplification product if the target nucleic acid sequence is present in the sample, and
- b) detecting the amplification product,
- wherein the amplification reagents comprise a modified nucleoside triphosphate having a photocleavable moiety and having a structure of:
- where Base is a purine or pyrimidine base or an analog.
A method of amplifying a target nucleic acid sequence using amplification reagents wherein the amplification reagents comprise a modified nucleoside triphosphate having a photocleavable moiety and having a structure of:
- where Base is a purine or pyrimidine base or an analog.
A method of reducing or preventing non-specific amplification of nucleic acid during an amplification reaction comprising providing amplification reagents that are used to amplify a target nucleic acid, wherein the amplification reagents comprise a modified nucleoside triphosphate having a photocleavable moiety and having a structure of:
- where Base is a purine or pyrimidine base or an analog.
A modified nucleoside triphosphate having a photocleavable moiety and having a structure of:
- where Base is a purine or pyrimidine base or an analog.
Dependencies are inferred from the claim text.