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AssigneesToyo Kohan Co., Ltd., Yamaguchi University
InventorsToshiya TSUDA, Shuichi KAMEI, Mitsuyoshi OBA, Hirofumi YAMANO, Ryouichi TSUNEDOMI, Shoichi HAZAMA, Hiroaki NAGANO
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In the case of using a blocking nucleic acid to prevent non-specific hybridization of a target nucleic acid with a nucleic acid probe, further excellent efficiency of detecting the target nucleic acid is achieved. A buffer composition used in hybridization of a target nucleic acid with a nucleic acid probe, wherein the buffer composition for hybridization contains a blocking nucleic acid comprising a nucleotide sequence complementary to a region comprising at least a non-detection target nucleotide in a non-target nucleic acid, in a concentration of one or more times higher than the concentration of a nucleic acid in a nucleic acid mixture consisting of the target nucleic acid and the non-target nucleic acid.
A buffer composition used in hybridization of a target nucleic acid comprising a detection target nucleotide with a nucleic acid probe comprising a nucleotide sequence complementary to a region comprising at least the detection target nucleotide in the target nucleic acid, wherein
A method of hybridizing a target nucleic acid comprising a detection target nucleotide with a nucleic acid probe comprising a nucleotide sequence complementary to a region comprising at least the detection target nucleotide in the target nucleic acid, wherein
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