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US 2019/0161776 A1Published application
Method for Producing Objective Substance
AssigneeAjinomoto Co., Inc.
InventorsBenjamin Mijts, Christine Roche, Rekha Murali, Sayaka Asari, Keita Fukui, Miku Toyazaki, Keiko Noguchi
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Abstract
A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism that is able to produce the objective substance, which microorganism has been modified so that the activity of an enzyme involved in SAM cycle (SAM cycle enzyme) is increased.
Claims34
A method for producing an objective substance, the method comprising:
- producing the objective substance by using a microorganism having an ability to produce the objective substance,
- wherein the microorganism has been modified so that the activity of an S-adenosylmethionine cycle enzyme is increased as compared with a non-modified microorganism,
- wherein the S-adenosylmethionine cycle enzyme is selected from the group consisting of methionine adenosyltransferase, methionine synthase, 5,10-methylenetetrahydrofolate reductase, and combinations thereof, and
- wherein the objective substance is selected from the group consisting of:
- (X) metabolites the biosynthesis of which requires S-adenosylmethionine;
- (Y) L-methionine;
- and
- (Z) combinations thereof,
- provided that the objective substance is selected from the metabolites (X) when only the activity of methionine adenosyltransferase is increased.
6 more elements
The method according to claim 10, wherein:
- the metK gene encodes a protein selected from the group consisting of:
- (1a) a protein comprising the amino acid sequence of SEQ ID NO:
- 162,
- (1b) a protein comprising the amino acid sequence of SEQ ID NO:
- 162 that includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein has methionine adenosyltransferase activity, and
- (1c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 162, and wherein said protein has methionine adenosyltransferase activity;
- the metH gene encodes a protein selected from the group consisting of:
- (2a) a protein comprising the amino acid sequence of SEQ ID NO:
- 168 or 170,
- (2b) a protein comprising the amino acid sequence of SEQ ID NO:
- 168 or 170 that includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein has methionine synthase activity, and
- (2c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 168 or 170, and wherein said protein has methionine synthase activity;
- the metE gene encodes a protein selected from the group consisting of:
- (3a) a protein comprising the amino acid sequence of SEQ ID NO:
- 166,
- (3b) a protein comprising the amino acid sequence of SEQ ID NO:
- 166 that includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein has methionine synthase activity, and
- (3c) a protein comprising an amino acid sequence showing an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 166, and wherein said protein has methionine synthase activity;
- and
- the metF gene encodes a protein selected from the group consisting of:
- (4a) a protein comprising the amino acid sequence of SEQ ID NO:
- 172, 174, or 184,
- (4b) a protein comprising the amino acid sequence of SEQ ID NO:
- 172, 174, or 184 that includes substitution, deletion, insertion, and/or addition of 1 to 10 amino acid residues, and wherein said protein has 5,10-methylenetetrahydrofolate reductase activity, and
- (4c) a protein comprising an amino acid sequence having an identity of 90% or higher to the amino acid sequence of SEQ ID NO:
- 172, 174, or 184, and wherein said protein has 5,10-methylenetetrahydrofolate reductase activity.
26 more elements
Dependencies are inferred from the claim text.