Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
AssigneeDanisco US Inc.
InventorsCHRISTINA GUTIERREZ, Colin Mitchinson, Tom T. Huang, Bruce A. Diner, Paul Joseph Fagan, William D. Hitz
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Abstract
The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.
Claims129
A method for simultaneous saccharification and fermentation (SSF) comprising culturing a complete fermentation medium, said complete fermentation medium comprising at least one fermenting microorganism, at least one xylan-containing biomass, at least one cellulase, at least one hemicellulase, and at least one inverting β-xylosidase, for a period and under conditions suitable for producing a fermentation product and optionally recovering the fermentation product.
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A T. reesei cell, which has been engineered such that the native β-xylosidase gene has been deleted or that it has no detectable β-xylosidase activity.
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A composition comprising at least one fermenting microorganism, at least one xylan-containing biomass, at least one cellulase, at least one hemicellulase, and at least one inverting β-xylosidase.
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Dependencies are inferred from the claim text.