Amplified production of monoclonal antibodies

A synthetic gene circuit system, comprising:

• a first expression construct comprising a nucleic acid encoding tetracycline transactivator (tTA) operably linked to a ubiquitous promoter;
• a second expression construct comprising a nucleic acid encoding tetracycline transactivator (tTA) operably linked to a tetracycline responsive element (TRE);
• a third expression construct comprising a nucleic acid encoding a heavy chain component of an monoclonal antibody operably linked to a TRE;
• and
• a fourth expression construct comprising a nucleic acid encoding a light chain component of an monoclonal antibody operably linked to a TRE,
• wherein expression of tTA by the first expression construct stimulates expression of tTA by the second expression construct, wherein expression of tTA by the first and second expression constructs stimulates expression of the heavy chain component of the monoclonal antibody and the light chain component of the monoclonal antibody, and wherein expression of tTA by the second expression construct establishes a positive feedback loop, thereby amplifying expression of the heavy chain component of the monoclonal antibody and the light chain component of the monoclonal antibody.