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US 2019/0161750 A1Published application
High-Throughput Single Cell Barcoding
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Francois Vigneault
Life of this patent
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Abstract
Methods and compositions for high-throughput, single cell analyses are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.
Claims20
A method for high-throughput sequencing of nucleic acids from a plurality of biological samples comprising
- (a) generating at least two bar-codes attached to a solid support,
- (b) delivering each of the at least two bar-codes to individual samples of the plurality of biological samples,
- (c) sequencing a nucleic acid from at least two of the plurality of biological samples;
- and
- (d) correlating the nucleic acid sequence to a single sample of the plurality of biological samples through bar-code sequencing identification.
2 more elements
A method of preparing bar-code oligonucleotides on a solid support comprising steps of
- (i) attaching an initial template oligonucleotide to a solid support,
- wherein the initial template oligonucleotide comprises a nucleotide sequence encoding (a) a sequencing primer, (b) a bar-code, and (c) an annealing primer,
- (ii) saturating the solid support with a plurality of anchor primers, and
- (iii) performing emulsion PCR.
1 more element
A solid support comprising a plurality of oligonucleotides wherein each oligonucleotide comprises a nucleotide sequence encoding:
- (i) a sequencing primer,
- (ii) a bar-code, and
- (iii) an annealing primer which targets a nucleic acid of interest.
Dependencies are inferred from the claim text.