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AssigneePioneer Hi-Bred International, Inc.
InventorsANDREW MARK CIGAN, MATTHEW G KING, HAINING LIN, JOSHUA K YOUNG
Estimating the term…
Compositions and methods are provided for novel Cas9 systems, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The novel Cas9 systems described herein are derived from a CRISPR-Cas locus that differs from type II CRISPR-Cas loci, in that the novel CRISPR-Cas locus described herein contains multiple CRISPR arrays flanking a tracrRNA encoding region and cas9 gene and does not contain a cas1 gene and a cas2 gene. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.
-19. (canceled)
A synthetic composition comprising a non-Type II Cas9 endonuclease and a guide RNA, wherein the synthetic composition forms a complex that recognizes, binds to, and optionally nicks or cleaves all or part of a target sequence.
A method for editing a nucleotide sequence at a target site in the genome of a cell, the method comprising introducing into said cell at least one guide RNA and at least one non-Type II Cas9 endonuclease protein or a functional fragment or functional variant thereof, wherein the guide RNA and non-Type II Cas9 endonuclease can form a complex that is capable of recognizing, binding to, and optionally nicking or cleaving all or part of the target site;
Dependencies are inferred from the claim text.