Combinational use of mechanical manipulation and programin to generate pluripotent stem cells from somatic cells
AssigneeThe Chinese University of Hong Kong
InventorsKenneth Ka Ho LEE, Tommy Lok Man LO, Hoi Hung CHEUNG, Wai Yee CHAN
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Abstract
The present invention provides methods and compositions for inducing pluripotency in differentiated mammalian cells. In particular, the methods include mechanically aggregating the cells into discrete masses or embryoid-like bodies and treated them with a small molecule compound. Provided herein are the compositions of the compounds which are derived from programin (e.g., reversine).
Claims9
1-20. (canceled)
A compound of Formula II-IX having the any one of the following structures:
A mixture comprising differentiated cells and a compound of Formula II-IX having any one of the following structures:
Dependencies are inferred from the claim text.