Process for continuous cell culture of cancer cells and cancer stem cells
AssigneeGeorgetown University
InventorsMaria Laura Aventaggiati, Giuseppe Giaccone
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Abstract
The present invention is directed towards compositions and methods of culturing cancer cells, with the methods comprising culturing cancer cells in the presence a cell culture medium while inhibiting the activity of Rho kinase (ROCK) in the cells during culturing.
Claims21
1-26. (canceled)
A composition comprising fibroblast growth factor (FGF), epithelial growth factor (EGF), insulin growth factor-1 (IGF-1), insulin, progesterone, transferrin, putrescine, pyruvate, albumin, selenite, thiamine, glutathione, ascorbic acid, and at least one Rho kinase (ROCK) inhibitor.
A population of conditionally immortalized cancer stem cells (CSCs).
Dependencies are inferred from the claim text.