Method for inducing antigen specific cd8 positive t cells
AssigneeKyoto University
InventorsHiroshi KAWAMOTO, Takuya MAEDA, Kyoko MASUDA
Life of this patent
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Abstract
Provided is a method for inducing CD4−CD8+ T cells having an antigen specific cytotoxic activity from pluripotent stem cells, comprising the steps of: (1) differentiating pluripotent stem cells to give a cell culture comprising CD4−CD8− T cells and CD4+CD8+ T cells, (2) removing CD4−CD8− cells from the cell culture obtained in step (1), and (3) differentiating the CD4+CD8+ cells in the cell culture into CD4−CD8+ T cells.
Claims25
A method for inducing CD4−CD8+ T cells, comprising the steps of:
- (1) differentiating pluripotent stem cells to give a cell culture comprising CD4−CD8− T cells and CD4+CD8+ T cells,
- (2) removing CD4−CD8− cells from the cell culture obtained in step (1), and
- (3) differentiating the CD4+CD8+ cells in the cell culture into CD4−CD8+ T cells, wherein the CD8 antigen of the CD4−CD8+ T cells is a heterodimer composed of CD8α and CD8β chains.
A method for inducing CD4−CD8+ T cells, comprising the steps of:
- (1) differentiating pluripotent stem cells to give a cell culture comprising CD4−CD8− T cells and CD4+CD8+ T cells,
- (2) differentiating the CD4+CD8+ T cells in the cell culture obtained in (1) into CD4−CD8+ T cells under the presence of a substance that inhibits cytotoxic activity of CD4−CD8− T cells.
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The method according to 20, wherein the step of further proliferating the obtained CD4−CD8+ T cells is performed by directly activating any part of the activation pathway which occurs on stimulating a T cell receptor.
25-27. (canceled)
Dependencies are inferred from the claim text.