Differentiated cell production method and culture bag used therefor
AssigneesDaikin Industries, Ltd., Kyoto University
InventorsKozue KOMAZAWA, Tatsuya HIGUCHI, Shin KANEKO, Yutaka YASUI
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Abstract
A method for producing differentiated cells from pluripotent stem cells by an embryoid body (EB) method, including the steps of: (1) culturing pluripotent stem cells by using a culture bag having a perfluoropolymer on its inner surface to thereby form an embryoid body; and (2) inducing differentiation of the pluripotent stem cells contained in the embryoid body obtained in step (1) to thereby obtain differentiated cells.
Claims7
A method for producing differentiated cells from pluripotent stem cells by an embryoid body (EB) method, comprising the steps of:
- (1) culturing pluripotent stem cells by using a culture bag having a perfluoropolymer on its inner surface to thereby form an embryoid body;
- and
- (2) inducing differentiation of the pluripotent stem cells contained in the embryoid body obtained in step (1) to thereby obtain differentiated cells.
A method for inducing differentiation of pluripotent stem cells by an embryoid body (EB) method, comprising the steps of:
- (1) culturing pluripotent stem cells by using a culture bag having a perfluoropolymer on its inner surface to thereby form an embryoid body;
- and
- (2) inducing differentiation of the pluripotent stem cells contained in the embryoid body obtained in step (1).
The culture bag according to claim 6, wherein the perfluoropolymer is at least one perfluoropolymer selected from the group consisting of tetrafluoroethylene-hexafluoropropylene copolymers, tetrafluoroethylene-perfluoroalkyl vinyl ether copolymers, and tetrafluoroethylene-hexafluoropropylene-perfluoroalkyl vinyl ether copolymers.
The culture bag according to claim 6, wherein the pluripotent stem cells are induced pluripotent stem cells (iPS cells) or embryonic stem cells (ES cells).
Dependencies are inferred from the claim text.