Methods and devices for electroporation
OwnerJian Cheninventor
Life of this patent
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Abstract
An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells.
Claims25
An apparatus for electroporation of biological cells, comprising:
- a sample container having an insulator chamber for holding the cells, the sample container having a first electrode and a second electrode providing electrical connection for electroporation, the insulator chamber configured to contain at least one cell monolayer;
- and
- a pulse generator capable of generating a predetermined pulse for electroporation of the cells.
An apparatus for electroporation of biological cells, comprising a sample container for holding a sample of biological cells for electroporation, the sample container comprising:
- an insulator chamber that forms the body of the container to hold the cells, the insulator chamber having a plurality of sides;
- a first electrode;
- and
- a second electrode, wherein:
- the first electrode and the second electrode receive an electrical pulse from an electrical pulse generator to electroporate the cells;
- wherein the insulator chamber and the electrodes are able to seal the sample of biological cells within the sample container.
The apparatus of 12, further comprising:
- an ultra-high voltage pulse generator for electroporation of the biological cells in the insulator chamber through the electrodes.
A process for electroporation of biological cells, comprising the steps of:
- arranging the cells to form at least one cell monolayer in an insulator chamber of a sample container, the sample container having a first electrode and second electrode providing electrical connection for electroporation;
- treating the cells in the cell monolayer with a predetermined electrical pulse, the predetermined electrical pulse being generated by a pulse generator.
Dependencies are inferred from the claim text.