Anti-nerve growth factor antibodies and methods of preparing and using the same

A caninized antibody or a caninized antigen binding fragment thereof which is capable of specifically binding to canine nerve growth factor (NGF) and inhibiting the ability of canine NGF to bind to the p75 canine NGF receptor and/or TrkA canine NGF receptor, wherein the antibody or antigen binding fragment comprises:

• (a) a light chain comprising a light chain variable region comprising the amino acid sequence selected from the group consisting of SEQ ID NO:
• 1 and substitution variants thereof differing from SEQ ID NO:
• 1 only by one or more amino acid substitutions selected from:
• Q3V;
• T5M, T51;
• S7T;
• A9L, A9P;
• S12A;
• L13V;
• S14A, S14T;
• Q15P, Q15R;
• E16G, E16D;
• E17D;
• K18T, K18P, K18A, K18E, K18L;
• V19A;
• T20S;
• T22S, T22Y;
• C23Y;
• Y36F, Y361, Y36L;
• Q37R, Q371, Q37L;
• Q38H;
• K39R;
• P40A, P40S;
• G41D;
• A43S, A43P, A43T;
• K45Q, K45E, K45R;
• L46R, L46A, L46G, L46P, L46S;
• 148L;
• Y49E, Y49F, Y49N, Y9S, Y49V;
• G47A;
• V58A;
• P59S;
• S60D;
• F62L, F62V;
• S631;
• G64A;
• T69A;
• E70D;
• Y71F;
• S72T, S72R;
• L73F;
• T74K, T74R;
• N76S;
• L78E;
• S80P, S80A;
• E81D, E81G, E811, E81N;
• V83A, V83G, V83S, V83T;
• A84G;
• V851, V85L;
• F87Y;
• G97S;
• A100P, A100Q, A100T;
• G101E;
• T102P;
• K103Q, K103S;
• V104L, V104W;
• E105D, E105R;
• and
• L1061;
• and
• (b) a heavy chain comprising a heavy chain variable region comprising the amino acid sequence selected from the group consisting of SEQ ID NO:2 and substitution variants thereof differing from SEQ ID NO:
• 2 only by one or more amino acid substitutions selected from:
• E1D, E1G;
• V2E, V2G, V21, V2L, V2M;
• Q3A, Q3E, Q3H, Q3K, Q3L, Q3P, Q3R, Q3S, Q3V;
• L4P, L4V;
• V5A V5E, V5L, V5M;
• E6A, E6Q;
• S7F, S7L, S7T;
• G9E;
• D10G, D10A, D10E, D10N, D10T;
• L11Q, L11R, L11V, L11W;
• V12A, V121, V12M;
• N13Q, N13K, N13R;
• P14S, P14T;
• G15T, G15A, G15E;
• G16E, G16A;
• T17S, T17P;
• L18R;
• T19R, T19G, T19K, T19V;
• L20I, L20V;
• S21Y;
• V23A, V23E, V231, V23L;
• V24A, V241, V24G, V24S, V24T;
• S25G, S25P, S25T;
• G26P, G26R, G26T;
• F27P, F271, F27L, F27S, F27T, F27V;
• S28T, S28A, S28D, S281, S28L, S28M, S28N, S28P, S28R;
• L29F, L291, L29M, L29V;
• T30S, T30D, T30G, T30H, T30I, T30K, T30N, T30R, T30V;
• W36C;
• V37A, V37F, V371, V37L;
• Q39H, Q39L;
• A40D, A40G, A40P, A40S, A40T, A40V;
• L41P;
• G42E, G42L, G42R;
• R43K, R43A, R43C, R43G, R43M, R43Q;
• G44D, G44E, G44R, G44T, G44V;
• L45F, L45M, L45P;
• E46Q, E46D, E46H, E46L, E46P, E46R;
• W47C, W47F, W47L, W47M, W47S, W47Y;
• V48F, V481, V48L;
• G49A, G49L, G49S, G49T;
• R66Q;
• L67F, L77V;
• T68A, T681, T68S;
• 169L, I69M, 169T, 169V;
• T70S, T70A, T70F;
• R71K;
• D72E, D72N;
• T73N, T73D, T73G, T731, T73S;
• S74A, S74D, S74G, S74P, S74T, S74V;
• K75E, K75G, K75M, K75N, K75Q, K75R;
• S76N, S76D, S76H, S76K, S76R;
• T77A, 1771, T77M, T77S;
• V78L, V78A, V781, V78M;
• F79Y, F79H, F79S, F79T;
• L80I;
• K81Q, K81A, K81D, K81H, K81E, K81R;
• M82L;
• H83N, H83D, H83K, H83P, H83R, H83S, H83T;
• S84D, S84G, S84N, S84R, S84T;
• L85V;
• Q86R, Q86G, Q86I, Q86K, Q86S, Q86T;
• S87A, S87D, S87G, S87P, S87T, S87V;
• E88A, E88D, E88V;
• T90A, T90M, T90S;
• A91G, A91V;
• T92V, T92F, T921, T92L, T92K, T92L, T92M, T92Q;
• Y93H;
• Y94F, Y94H;
• A96C, A96G, A96L, A96M, A96R, A96S, A96T, A96V;
• R97K, R97A, R97D, R97E, R97G, R971, R97L, R97M, R97N, R97P, R97Q, R97S, R97T, R97V;
• W112L;
• G113A, G13S;
• Q114D, Q114H, Q114P, Q114R;
• T116A, T16I, T116N, T116S;
• L117S, L117P, L117Q, L117R;
• V1181, V118L, V118P;
• T119A, T119F, T1191, T119L, T119P, T119S, T119Y;
• V120A;
• S121A, S121C, S121P, S121T;
• and
• S122L, S122P.