Isolation device and isolation method
AssigneesShenzhen Wellsim Biomedical Technologies, Wellsim Biomedical Technologies, Inc
InventorYU-CHAO CHEN
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Abstract
An isolation device of target particles from a liquid sample includes an isolation chip, a vacuum unit, and a frequency converting unit. The isolation chip includes a sample reservoir, a first chamber, and a second chamber. The first chamber and the second chamber define a first outlet and a second outlet, respectively. The vacuum unit is connected to the first outlet and the second outlet. The frequency converting unit causes the vacuum unit to generate negative pressures in the first chamber and the second chamber alternately.
Claims18
An isolation device of target particles from a liquid sample, comprising:
- an isolation chip comprising:
- a sample reservoir configured for receiving the liquid sample;
- a first filtration membrane comprising pores of sizes smaller than sizes of the target particles;
- a second filtration membrane comprising pores of sizes smaller than the sizes of the target particles;
- a first chamber connected to the sample reservoir through the first filtration membrane, the first chamber defining a first outlet;
- and
- a second chamber connected to the sample reservoir through the second filtration membrane, the second chamber defining a second outlet;
- a vacuum unit connected to each of the first outlet and the second outlet;
- and
- a frequency converting unit causing the vacuum unit to generate negative pressures in the first chamber and the second chamber alternately.
The isolation device of FIG. 4, further comprising a detector and a controller, wherein the detector is adapted to detect a liquid level of the liquid sample in the sample reservoir, the controller is electrically connected to each of the detector and the frequency converting unit, the controller is configured to obtain the liquid level detected by the detector, and determine whether isolation of the liquid sample is finished according to the obtained liquid level and a first preset amount of the liquid sample, when isolation of the liquid sample is determined to be finished, the controller is configured to control the frequency converting unit to stop generating negative pressures in the first chamber and the second chamber.
An isolation method of target particles from liquid sample, comprising:
- providing an isolation chip, wherein the isolation chip comprises a sample reservoir, a first filtration membrane, a second filtration membrane, a first chamber, and a second chamber, a first filtration membrane comprises pores of sizes smaller than sizes of the target particles, the second filtration membrane comprises pores of sizes smaller than the sizes of the target particles, the first chamber is connected to the sample reservoir through the first filtration membrane, the first chamber defining a first outlet, the second chamber is connected to the sample reservoir through the second filtration membrane, the second chamber defining a second outlet;
- adding the liquid sample to the sample reservoir;
- evacuating the first chamber through the first outlet to generate a negative pressure in the first chamber;
- stopping vacuuming of the first chamber;
- evacuating the second chamber through the second outlet to generate a negative pressure in the second chamber;
- and
- stopping vacuuming of the second chamber.
An isolation device of target particles from a liquid sample, comprising:
- an isolation chip comprising a sample reservoir, a first filtration membrane, a second first filtration membrane, a first chamber, and a second chamber, the first filtration membrane comprising pores of sizes smaller than sizes of the target particles, the second filtration membrane comprising pores of sizes smaller than the sizes of the target particles, the first chamber connected to the sample reservoir through the first filtration membrane, the first chamber defining a first outlet, the second chamber connected to the sample reservoir through the second filtration membrane, the second chamber defining a second outlet;
- a vacuum unit connected to each of the first outlet and the second outlet;
- and
- a frequency converting unit electrically connected to the vacuum unit;
- a liquid provider;
- a controller;
- and
- a storage device coupled to the controller and storing one or more programs to be executed by the controller, wherein when executed by the controller, the one or more programs cause the controller to:
- control the liquid provider to add the liquid sample to the sample reservoir;
- and
- control the vacuum unit to generate negative pressures in the first chamber and the second chamber alternately through the frequency converting unit.
Dependencies are inferred from the claim text.