Isolation chip and manufacturing method of the same
AssigneesShenzhen Wellsim Biomedical Technologies, Wellsim Biomedical Technologies, Inc
InventorYU-CHAO CHEN
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Abstract
A device, system, and method for separating, concentrating, and isolating target particles from a bioliquid sample includes a sample reservoir, a first filtration membrane, a second filtration membrane, a first chamber, and a second chamber. The first chamber with first outlet is connected to the sample reservoir through the first filtration membrane. The second chamber with second outlet is connected to the sample reservoir through the second filtration membrane. Negative pressures are applied alternately to the two filtration membranes to isolate target particles, clogging of the membranes being prevented by the same alternating but opposite-phase positive pressures.
Claims14
An isolation chip for isolation and purification of target particles from a liquid sample, comprising:
- a sample reservoir configured for receiving the liquid sample;
- a first filtration membrane comprising pores of sizes smaller than sizes of the target particles;
- a second filtration membrane comprising pores of sizes smaller than the sizes of the target particles;
- a first chamber connected to the sample reservoir through the first filtration membrane, the first chamber defining a first outlet connecting the first chamber to an ambient environment;
- and
- a second chamber connected to the sample reservoir through the second filtration membrane, the second chamber defining a second outlet connecting the second chamber to the ambient environment.
A manufacturing method of an isolation chip for isolation and purification of target particles from a liquid sample, the manufacturing method comprising:
- providing a reservoir substrate, a first inner cover, a second inner cover, a first side cover, a second side cover, a first filtration membrane, and a second filtration membrane, the first side cover defining a first outlet, the second side cover defining a second outlet, the first filtration membrane and the second filtration membrane comprising pores of sizes smaller than sizes of the target particles;
- forming a first chamber by connecting the first side cover to a side of the first inner cover, the first outlet connecting the first chamber to an ambient environment;
- connecting the first filtration membrane to the first inner cover;
- forming a sample reservoir by successively connecting the reservoir substrate and the second inner cover to a side of the first inner cover facing away from the first side cover, thereby forming a flow path from the first chamber being to the sample reservoir through the first filtration membrane;
- connecting the second filtration membrane to the second inner cover;
- and
- forming a second chamber by connecting the second side cover to a side of the second inner cover facing away from the reservoir substrate, thereby forming a flow path from the second chamber to the sample reservoir through the second filtration membrane, and the second outlet connecting the second chamber to the ambient environment.
A manufacturing method of an isolation chip for isolation and purification of target particles from a liquid sample, the manufacturing method comprising:
- providing a first side cover comprises a first protruding block and a first chip base, and a second side cover comprising a second protruding block and a second chip base, wherein the first protruding block divides the first side cover into a first cover portion and a second cover portion which are at two opposite sides of the first protruding block, the second protruding block divides the second side cover into a third cover portion and a fourth cover portion which are at opposite sides of the second protruding block, the first chip base is connected to an end of the first side cover and faces the first protruding block, the second chip base is connected to an end of the second side cover and faces the second protruding block, the first side cover defining a first outlet, the second side cover defining a second outlet;
- providing a first filtration membrane and a second filtration membrane, each of the first filtration membrane and the second filtration membrane having pores of sizes smaller than sizes the target particles;
- connecting the first filtration membrane between the first protruding block and the first chip base;
- connecting the second filtration membrane between the second protruding block and the second chip base;
- forming a sample reservoir defined between the first cover portion, the third cover portion, the first protruding block, and the second protruding portion;
- forming a first chamber defined between the second cover portion, the first filtration membrane, and the first chip base;
- and
- forming a second chamber defined between the fourth cover portion, the second filtration membrane, and the second chip base;
- wherein the first chamber is connected to the sample reservoir through the first filtration membrane, and the second chamber being connected to the sample reservoir through the second filtration membrane.
Dependencies are inferred from the claim text.