Compositions and methods to treat aids
AssigneeUniversity of Massachusetts
InventorsShan Lu, Shixia Wang
Life of this patent
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Abstract
Polyvalent, primary isolate nucleic acid compositions for inducing an immune response against HIV are disclosed. The compositions and methods described herein are for the use of a nucleic acid composition that encodes one or more different HIV envelope glycoproteins. The synthetic, codon-optimized DNAs encoding one or more HIV proteins are a combination of different nucleic acids, such as DNA plasmids, generated from primary isolate DNA of different HIV major group genetic clades and/or different proteins. HIV polypeptide compositions for inducing an immune response against HIV are also disclosed. Methods for using the polypeptide compositions before, at the same time as, and/or after administration of the DNA compositions are provided.
Claims19
(canceled)
A composition comprising at least two synthetic nucleic acid molecules selected from the group consisting of:
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:1;
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:3;
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:5;
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:7;
- and
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:9.
(canceled)
A composition comprising at least two polypeptides selected from the group consisting of:
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 2;
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 4;
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 6;
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 8;
- and
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:
- 10.
(canceled)
A method of inducing an immune response against human immunodeficiency virus (HIV) or an HIV epitope in a subject, the method comprising administering to the subject at least one of the following:
- (1) a nucleic acid composition comprising at least three synthetic nucleic acid molecules selected from the group consisting of:
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:1;
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:3;
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:5;
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:7;
- and
- a synthetic nucleic acid molecule comprising a nucleotide sequence that has at least 90% identity to the nucleotide sequence of SEQ ID NO:9;
- and
- (2) a polypeptide composition comprising at least three polypeptides selected from the group consisting of:
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:2;
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:4;
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:6;
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:8;
- and
- a polypeptide comprising an amino acid sequence that has at least 90% identity to the amino acid sequence of SEQ ID NO:10;
- wherein the nucleic acid composition and/or the polypeptide composition are administered in an amount sufficient to induce an immune response against HIV or an HIV epitope in the subject.
(canceled)
Dependencies are inferred from the claim text.