Loading patent…
AssigneeMississippi State University
InventorsAttila Karsi, Mark Lawrence, Hossam Abdelhamed
Estimating the term…
A live attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional evpB protein and a method of using the same to protect fish against infection from virulent Edwardsiella ictaluri. The methods and compositions for protecting fish against infection from virulent Edwardsiella ictaluri comprising administering to a fish a therapeutically effective amount of an attenuated Edwardsiella ictaluri bacterium lacking a viable gene encoding a functional protein selected from the group consisting of evpB, fur and hfq. The bacterium may include an insertion and/or deletion mutation in the gene. The fish include catfish, preferably catfish fingerling or a catfish fry. The composition may be delivered via immersion delivery, an injection delivery, an oral delivery, or combinations thereof.
A modified bacterium, the modified bacterium lacking a viable gene encoding a functional EvpB protein by having a deletion of the coding sequence of the evpB gene.
A vaccine for protecting fish against Edwardsiella ictaluri infection comprising an immunogenically effective amount of an attenuated Edwardsiella ictaluri bacterium, the bacterium lacking a viable gene encoding a functional protein by having deletions of the coding sequence of a gene selected from the group consisting of ferric uptake regulator (Fur) and hfq.
A composition comprising an amount of live attenuated Edwardsiella ictaluri bacterium sufficient for protecting fish against infection from virulent Edwardsiella ictaluri, the bacterium lacking a viable gene encoding a functional evpB protein by having a deletion of the coding sequence of evpB and fur genes, and one or more of the group consisting of a pharmaceutically-acceptable vehicle, a carrier, and an excipient.
A method for protecting fish against infection from virulent Edwardsiella ictaluri comprising:
Dependencies are inferred from the claim text.