US 2019/0160109 A1 · Notochordal cell matrix as a bioactive lubricant for the osteoarthritic joint

Abstract

A solubilized notochordal cell matrix powder dissolved in a carrier solvent or formed as a gel is provided. The notochordal cell matrix powder originates from lyophilized and treated porcine nucleus pulposus tissue containing notochordal cells. The powder contains less than 20% of porcine nucleid acids, and the powder contains a substantially unchanged amount of porcine protein content compared to the originating porcine nucleus pulposus tissue. The solubilized notochordal cell matrix powder is capable of stimulating native or stem cells to proliferate and produce a significant increase in glycosaminoglycans and type-II collagen matrix. Embodiments of the invention can be used as a lubricant with regenerative potential for application in the osteoarthritic joint.

Claims5

Dependent claims

A method of making a notochordal cell matrix as a bioactive lubricant for an osteoarthritic joint, comprising:

(a) lyophilizing porcine nucleus pulposus tissue containing notochordal cells to destroy cells within the tissue and to make a dry and brittle tissue;
(b) treating the dry and brittle tissue to remove cellular and nucleic acid remnants, wherein the treatment results in at least 80% removal of porcine nucleic acids from the porcine nucleus pulposus tissue while substantially maintaining porcine protein content within the porcine nucleus pulposus tissue;
(c) lyophilizing the treated material and pulverizing the treated material into a notochordal cell matrix powder;
and
(d) solubilizing the notochordal cell matrix powder by dissolving the notochordal cell matrix powder into a solution or a gel.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/261,771Filed 30 Jan 2019

Priority

11 Jan 2016

Published

30 May 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

A61K 35/32
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61K 9/0085
Galenical forms characterised by the site of application: brain, e.g. brain implants; Spinal cord
A61K 9/06
Medicinal preparations characterised by special physical form: ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
A61K 9/08
Medicinal preparations characterised by special physical form: solutions

Family

Application62/277,032Us provisional application · filed 11 Jan 2016

Application62/623,672Us provisional application · filed 30 Jan 2018

Application16/068,752Continuation in part · filed 9 Jul 2018

Application16/261,771Continuation in part

US 11,607,473 B2Related patent

US 2019/0022278 A1Published application

US 2019/0160109 A1This patent

From the US filing history. Foreign family members aren’t listed.

Notochordal cell matrix as a bioactive lubricant for the osteoarthritic joint

Life of this patent

Notochordal cell matrix as a bioactive lubricant for the osteoarthritic joint

Life of this patent

Abstract

Claims5